Ex. 19: Urea Hydrolysis (Urease Test)

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Ex. 19: Urea Hydrolysis (Urease Test) Objectives ??

Work in a team of two students Day 1 Materials needed per team of two students: Urea agar slants (3) Pure cultures needed per table: Proteus vulgaris Escherichia coli Klebsiella pneumoniae Work in a team of two students

urea This test checks if bacterium has enzyme urease Medium contains urea, phenol red, peptones, glucose, potassium phosphate Mainly used to differentiate Proteus from other Enterobacteriaceae

Table 19-1: Possible Results for Urease Test Interpretation Symbol Up to 24 hours 24 hours to 6 days All pink Rapid urea hydrolysis; strong urease production + Partially pink All pink or partially pink Slow urea hydrolysis; weak urease production w + Orange or yellow No urea hydrolysis; urease is absent ­

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