Lecture 16 Chemical Reaction Engineering (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors.

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Presentation transcript:

Lecture 16 Chemical Reaction Engineering (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.

Web Lecture 16 Class Lecture 25 – Thursday Bioreactors Monod Equation Yield Coefficients Washout

Enzymes - Urease Review Last Lecture The corresponding mechanism is: A given enzyme can only catalyze only one reaction. Urea is decomposed by the enzyme urease, as shown below. The corresponding mechanism is: Michaelis Menten Equation

Bioreactors Nutrient (S) + + Products cells more cells

Bioreactors

Batch Bioreactor

Phases of Cell Growth and Division

Bioreactors inoculum t=0 time  Cells + Substrate  More Cells + Product

Bioreactors Phases of bacteria growth: I. Lag II. Exponential III. Stationary IV. Death b) Monod growth rate law: Exponential Growth Phase Death Phase Stationary Phase Lag Phase time lnCc

Bioreactors 1) Mass Balances Accumulation = [In] - [Out] + [Growth] - [Death]

Bioreactors Rate Laws: Cp*= Product concentration at which all metabolism ceases

Bioreactors 3) Stoichiometry A) Yield Coefficients B) Maintenance

Bioreactors 3) Stoichiometry Rate of Substrate Consumption Can’t separate substrate consumption used for cell growth from that used for product formations during exponenial growth.

Bioreactors v0 CS0 V=V0 v CC CS Volume=V 1) Mass Balance:

Bioreactors

Bioreactors 2) Rate Laws: 3) Parameters

Polymath Setup 1.) d(CC)/d(t)=-D*CC+(rg-rd) 2.) d(CS)/d(t)=-D*(CS0-CS)-Ysg*rg-m*CC 3.) d(CP)/d(t)=-D*CP+YPC*rg 4.) rg=(((1-(CP/Cpstar))**0.52)*mumax*(CS/(KS+CS))*CC 5.) D=0.2 6.) kd=0.01 11.) mumax=0.33 7.) rd=kd*CC 12.) YSC=12.5 8.) CS0=250 13.) KS=1.7 9.) YPC=5.6 10.) m=0.3

Bioreactors – Chemostats - CSTRs 1. Steady State - Neglect Death Rate and Cell Maintenance 2. Cell

Bioreactors – Chemostats - CSTRs 3. Substrate

Bioreactors – Chemostats - CSTRs CSTR Washout CC DW D Washout dilution rate

Maximum Product Flow Rate DCC DW D Dmaxprod How does this figure relate to drinking a lot of fluids when you have an infection or cold?

End of Web Lecture 16 End of Class Lecture 25