A Reply to “MNase-Sensitive Complexes in Yeast: Nucleosomes and Non-histone Barriers,” by Chereji et al. Slawomir Kubik, Maria Jessica Bruzzone, Benjamin.

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Presentation transcript:

A Reply to “MNase-Sensitive Complexes in Yeast: Nucleosomes and Non-histone Barriers,” by Chereji et al. Slawomir Kubik, Maria Jessica Bruzzone, Benjamin Albert, David Shore Molecular Cell Volume 65, Issue 3, Pages 578-580 (February 2017) DOI: 10.1016/j.molcel.2017.01.010 Copyright © 2017 Elsevier Inc. Terms and Conditions

Figure 1 Rapid Nuclear Depletion of TFs at RPG Promoters Does Not Alter the MNase Sensitivity of Particles Upstream of the TSS-Associated +1 Nucleosome Plots of nucleosome occupancy at category I RPGs (Knight et al., 2014) obtained using low (light green) or high (dark green) MNase concentrations in the presence (solid line) or absence (dashed line) of specific TFs: Sfp1 (left) or Hmo1 and Fhll (right), which were depleted using the anchor away method (Haruki et al., 2008). The plots are centered on the dyads of −1 fragile nucleosomes (FNs). Molecular Cell 2017 65, 578-580DOI: (10.1016/j.molcel.2017.01.010) Copyright © 2017 Elsevier Inc. Terms and Conditions