Primary human MNCs and MDMs are targets of ZIKV infection.

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Primary human MNCs and MDMs are targets of ZIKV infection. Primary human MNCs and MDMs are targets of ZIKV infection. Isolated human primary MNCs and MDMs (2 × 106 cells each) were infected with ZIKV at an MOI of 10 and harvested at 24 and 72 hpi. (A and B) Flow cytometry gating on monocytes (MNCs) (A) and MDMs (B). Gating for positive infection was set using the mock-infected samples. For the dot plots, cells positive for ZIKV antigen (Ag) are shown in red. For the histogram, ZIKV-infected samples (red) were overlaid on mock-infected samples (black). Gating of live cells was performed on single cells. SSC, side scatter; FSC, forward scatter. (C and D) Compiled results for infection (ZIKV Ag) (C) and viral load detected in MNCs and MDMs obtained from five healthy donors (D). All data are presented as means ± standard deviations. **, P < 0.05, by Mann-Whitney U test, two-tailed. Viral load data were not statistically significant between 24 and 72 hpi in MNCs by Mann-Whitney U test, two-tailed. Fok-Moon Lum et al. mSphere 2018; doi:10.1128/mSphereDirect.00120-18