Functional properties of pRb.

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Functional properties of pRb. Functional properties of pRb. (A) Functional consequences of pRb acetylation on E2F‐1 activity. Expression vectors encoding wild‐type (WT) pRb, pRb RR873/874 or pRb QQ873/874 (1 μg) were coexpressed with E2F‐1 (200 ng), and the effect on cyclin E‐luciferase (200 ng) was measured. Transfection into U2OS cells was carried out as described previously (Chan et al, 2001). (B) Levels of wild‐type pRb and 763–928 derivatives in U2OS cells transfected as described in (A). (C) Intracellular location of pRb and 763–928 derivatives in U2OS cells (transfected with 1 μg expression vector and treated as described). The 4,6‐diamidino‐2‐phenylindole (DAPI) staining shows the location of nuclei. (D) Model for the influence of DNA‐damage‐dependent pRb acetylation on E2F‐1 activity. The results presented in this study suggest that residues KK873/874 influence the interaction between E2F‐1 and the pRb carboxy‐terminal E2F‐1‐specific binding domain. Acetylation of KK873/874 activated through the DNA damage response liberates E2F‐1 and theoretically allows E2F‐1 activity to be regulated independently of generic E2F binding to the pRb pocket domain. Douglas Markham et al. EMBO Rep. 2006;7:192-198 © as stated in the article, figure or figure legend