by Christina Bartholdy, Ole Marker, and Allan Randrup Thomsen Migration of activated CD8+ T lymphocytes to sites of viral infection does not require endothelial selectins by Christina Bartholdy, Ole Marker, and Allan Randrup Thomsen Blood Volume 95(4):1362-1369 February 15, 2000 ©2000 by American Society of Hematology

Surface phenotype of LCMV-activated CD8+ T cells Surface phenotype of LCMV-activated CD8+ T cells.C57BL/6 mice were infected iv with 103 LD50 of LCMV, and on day 8 pi splenocytes were harvested (Inf). Surface phenotype of LCMV-activated CD8+ T cells.C57BL/6 mice were infected iv with 103 LD50 of LCMV, and on day 8 pi splenocytes were harvested (Inf). Cells were stained with anti-CD8-PE, anti-VLA-FITC, and either anti-IFN-γ, anti-L-selectin, or anti-PSGL-1. Spleen cells from uninfected mice were used as control (Con). Results representative of analyses of 3 to 5 mice/group are depicted. Christina Bartholdy et al. Blood 2000;95:1362-1369 ©2000 by American Society of Hematology

LCMV-specific TC activity in E/P-sel −/−, E-sel −/−, and wild-type (wt) C57BL/6 mice 8 days after iv infection.Mice were infected iv with 103 LD50 of LCMV, and splenic TC activity was assayed using MC57G cells infected 48 hours previously LCMV. Uninfected M... LCMV-specific TC activity in E/P-sel −/−, E-sel −/−, and wild-type (wt) C57BL/6 mice 8 days after iv infection.Mice were infected iv with 103 LD50 of LCMV, and splenic TC activity was assayed using MC57G cells infected 48 hours previously LCMV. Uninfected MC57G cells, assayed only at effector:target (E:T) ratio 80:1 and 40:1 were used as negative controls. Medians and ranges of 3 mice per group are depicted. Christina Bartholdy et al. Blood 2000;95:1362-1369 ©2000 by American Society of Hematology

Absence of selectins do not influence the time course or outcome of ic infection.E/P-sel −/−, E-sel −/−, and wild-type (wt) mice were infected ic with 103 LD50 of LCMV, and mortality was registered. Absence of selectins do not influence the time course or outcome of ic infection.E/P-sel −/−, E-sel −/−, and wild-type (wt) mice were infected ic with 103 LD50 of LCMV, and mortality was registered. Groups consisted of 6 to 7 mice. Christina Bartholdy et al. Blood 2000;95:1362-1369 ©2000 by American Society of Hematology

Time course of footpad swelling in E/P-sel −/− and wild-type (wt) mice after local LCMV infection.Mice were infected with 103 LD50 of LCMV in the right hind footpad, and footpad swelling was measured from day 6 to 17 pi. Time course of footpad swelling in E/P-sel −/− and wild-type (wt) mice after local LCMV infection.Mice were infected with 103 LD50 of LCMV in the right hind footpad, and footpad swelling was measured from day 6 to 17 pi. Groups consisted of 6 mice; medians and ranges are presented. Christina Bartholdy et al. Blood 2000;95:1362-1369 ©2000 by American Society of Hematology

Time course of peptide-induced footpad swelling in infected E/P-sel −/−, E-sel −/−, and wild-type (wt) mice.Mice were infected iv with 103 LD50 of LCMV and challenged in the right hind footpad with LCMV GP 33-41 (50 μg/mL, 30 μL) on day 8 pi; this peptide r... Time course of peptide-induced footpad swelling in infected E/P-sel −/−, E-sel −/−, and wild-type (wt) mice.Mice were infected iv with 103 LD50 of LCMV and challenged in the right hind footpad with LCMV GP 33-41 (50 μg/mL, 30 μL) on day 8 pi; this peptide represents an immunodominant class I–restricted peptide in H-2b mice. Groups consisted of 4 to 6 mice; medians and ranges are presented. * denotesP < .05 relative to wild-type mice. Christina Bartholdy et al. Blood 2000;95:1362-1369 ©2000 by American Society of Hematology

Time course of adoptively transferred virus-specific footpad swelling Time course of adoptively transferred virus-specific footpad swelling.(A) In E/P-sel −/−, E-sel −/−, and wild-type (wt) recipients. Time course of adoptively transferred virus-specific footpad swelling.(A) In E/P-sel −/−, E-sel −/−, and wild-type (wt) recipients. (B) In P-sel/ICAM-1 −/−, P-sel −/−, and wild-type recipients. (C) In ICAM-1 −/− mice given donor cells preincubated with soluble VCAM-1-Ig chimeric protein (sVCAM) or human Ig for control. Mice were inoculated in their right footpad with 103 LD50of LCMV. Four hours later they were injected with 5 × 107adherent cell-depleted splenocytes from wild-type donors, infected 8 days earlier with the same dose of virus iv. Groups consisted of 4 to 6 mice; medians and ranges are presented. * denotesP < .05 relative to wild-type mice. Christina Bartholdy et al. Blood 2000;95:1362-1369 ©2000 by American Society of Hematology

Organ virus titers in E/P-sel −/−, E-sel −/−, and wild-type (wt) mice infected iv with 103LD50 of LCMV Traub.Organs (splen, liver, and lungs) were harvested on the indicated days relative to virus inoculation and virus titers were determined. Organ virus titers in E/P-sel −/−, E-sel −/−, and wild-type (wt) mice infected iv with 103LD50 of LCMV Traub.Organs (splen, liver, and lungs) were harvested on the indicated days relative to virus inoculation and virus titers were determined. Points represent individual mice. Christina Bartholdy et al. Blood 2000;95:1362-1369 ©2000 by American Society of Hematology