Isolating DNA from Bacterial Cells.

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Presentation transcript:

Isolating DNA from Bacterial Cells

The Central Dogma of Biology The Central Dogma of Biology. Proteins are produced when genes on a DNA molecule are transcribed into mRNA, and mRNA is translated into the protein code. This is called “gene expression.” At any given moment, only a relatively small amount of DNA in a cell is being expressed.

It is DNA Structure that allows its isolation from solution DNA Structure. The nucleotides in one chain of the helix face one direction, while those in the other strand face the other direction. Each nucleotide contains a sugar molecule, a phosphate group, and a nitrogenous base. Nitrogenous bases from each strand bond to each other in the center through H-bonds. The H-bonds are rather weak; therefore, the two strands of DNA separate easily in high temperatures.