PCR analysis of platelet cDNA preparations

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by Mark T. Boyd, Brian Foley, and Isadore Brodsky

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binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.

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Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,

Analysis of CD151 and CD147 as potential prostate cancer biomarkers.

Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial cells. Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial.

Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.

Plasma CEP adducts and autoantibodies by donor age.

Illustration of matched (FL-IFN-γR2/GFP and IFN-γR1/EBFP) and mismatched (IFN-γR1/EBFP and FL-IL-10R2/GFP) pairs of receptors. Illustration of matched.

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Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,

Top-down protein identification.

Distributions of the ELDP values and Mascot scores for all protein identifications.a, frequency of ELDP value returned by correct (gray bars) and incorrect.

Visual validation of the computational outputs.

Novel p53 target genes identified by RNA-Seq, pSILAC and ChIP-Seq.

Assay of NOS activity. Assay of NOS activity. Box show total NOS activity, the calcium-dependent activity of the constitutive isoforms of NOS (eNOS and.

Expression of apocrine markers at various stages of apocrine carcinoma progression. Expression of apocrine markers at various stages of apocrine carcinoma.

Relative abundance of proteins identified in MALDI IMS

Comparison of ROC plots for the PMF quality metrics using test dataset 2 (44 C. difficile proteins).a, ROC curves for coverage (open squares), MC (solid.

Bottom-up proteomic characterization of MALDI IMS samples.

Success rates in validation of antibodies from external providers

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Degree of glycosylation of human milk LF from individual donors across lactation. Degree of glycosylation of human milk LF from individual donors across.

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Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.

High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1.

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Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling.

Plot of the deviation of the predicted pI value of every peptide spectrum from the average pI calculated for each fraction for validated (a) and non-validated.

Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.

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Changes in protein expression during distinct stages of NK cell differentiation. Changes in protein expression during distinct stages of NK cell differentiation.

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Model of the change in receptor structure on engagement of the ligand IFN-γ. Model of the change in receptor structure on engagement of the ligand IFN-γ.

Presentation transcript:

PCR analysis of platelet cDNA preparations PCR analysis of platelet cDNA preparations.A, PCR was performed to detect a platelet marker (CD151) and a nucleated cell maker (T cell receptor). PCR analysis of platelet cDNA preparations.A, PCR was performed to detect a platelet marker (CD151) and a nucleated cell maker (T cell receptor). Lanes 1–5, cDNA from individual platelet preparations. Lane 6, negative (no template) control. Lane 7, cDNA prepared from whole blood. Platelet RNA preparations are free of contaminating cell markers. B, PCR products from the 5′ end of GP IIb and GP IIIa were routinely detected in platelet cDNA synthesized by priming with oligo-dT at the 3′ end, demonstrating intact RNA up to 4 kb long. Lanes 1–11, individual platelet cDNA preparations. Lane 12, no template control. C, The entire coding region of Gas6, amplified from human platelet cDNA. Lane 1, platelet cDNA. Lane 2, no template. Lane 3, molecular weight markers, size indicated in kbp. J. P. McRedmond et al. Mol Cell Proteomics 2004;3:133-144 © 2004 The American Society for Biochemistry and Molecular Biology