LEUKOPOIESIS I WAYAN SUGIRITAMA http://sugiritama.blogspot.com sugiritama@gmail.com

LEUKOPOIESIS “Formation and maturation of white blood cells” Granulocytopoiesis Agranulocytopoiesis Monocytopoiesis Lymphoctye maturation

Mature White Blood Cells

NEUTROPHILS

EOSINOPHILS

BASOPHILS

LYMPHOCYTES

MONOCYTES

BONE MARROW : The Site of Leucopoiesis Vascular compartment Extensive network of sinusoids . Hemopoietic compartment Islands of hematopoietic cells (Hemopoietic cells in various stage of maturation )

HEMOPOIETIC GROWTH FACTORS Table 10-6 Gartner colour text histology page 242 Regulated the hemopoiesis Produce by spesific cells Acts on spesific stem cells, progenitor cells, and precursor cells The route to deliver growth factor : Via blood stream Secrete near the hemopoietic cells Direct cell-cell contact Induced rapid mitosis or and differentiation Most ot them are glycoproteins

GRANULOCYTOPOIESIS

GRANULOCYTOPOIESIS Formation of the granulocytes (neuthrophil, eosinophil, and basophil) All of Granulocyte progenitor cell derive from CFC-S Granulocyte Progenitor : CFU-Eo : eosinophil lineage CFU-Ba : Basophil lineage CFU-GM CFU-G : Neutrophil line CFU-M : monocyte line

Maturation of Granulocyte Granulocyte maturation divide into six stages Structural Changes : A decreased in cell size Condensation of nuclear chromatin Changes in nuclear shape An accumulation of cytoplasmic granule

Maturation of Granulocyte (Table 10-8 Gartner colour text histology page 242)

GRANULOCYTOPOIESIS Mature granulocyte leave the bone marrow then enter the circulation→ connective tissue

Monocytopoiesis

MONOBLAST Large cell (15-25 ) Nucleus oval Cytoplasm without granule or few azurophlic granule Differentiate to the Promonocyte

PROMONOCYTE 16-18 µm in diameter Kidney shape nucleus Cytoplasm contain azurophilic granule Divide twice in the course of their development into monocytes

MONOCYTE ON BONE MARROW Mature monocytes enter the bloodstream → circulate. Monocyte enter the connective tissues, where they mature into macrophages.

LYMPHOPOIESIS

LYMPHOPOIESIS CFU-Ly divides form the CFU-LyB and CFU-LyT CFU-LyB migrate to “bursa-equivalent location”, divided → B lymphocytes CFU-LyT undergo mitosis migrate to the Thymus → T lymphocytes

LYMPHOBLAST

LYMPHOCYTE ON BONE MARROW

LYMPHOCYTE

CONCLUSION Leucocytosis is formation of WBC, occurs in Bone Marrow Granulocyte and monocyte derive from Myeloid stem cells, whereas limfocyte from Lymphoid Stem cell Leucocytosis involved the series of mitosis and differentiation Leucocytosis are regulated by several growth factor and Interleukin

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