Expression and localization of the novel and highly conserved gametocyte-specific factor 1 during oogenesis and spermatogenesis Stephan P. Krotz, M.D.,

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Presentation transcript:

Expression and localization of the novel and highly conserved gametocyte-specific factor 1 during oogenesis and spermatogenesis Stephan P. Krotz, M.D., Daniel J. Ballow, B.A., Youngsok Choi, Ph.D., Aleksandar Rajkovic, M.D., Ph.D. Fertility and Sterility Volume 91, Issue 5, Pages 2020-2024 (May 2009) DOI: 10.1016/j.fertnstert.2008.05.042 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Gtsf1 expression in wild-type (WT) and Nobox-deficient female mice. Gtsf1 expression is down-regulated 20-fold in Nobox-deficient female mice compared with wild-type female mice (P≤.011). Fertility and Sterility 2009 91, 2020-2024DOI: (10.1016/j.fertnstert.2008.05.042) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A–C) Gtsf1 RNA and protein expression. Equal amounts of total RNA from 11 different adult tissues as indicated above (A) and from various embryonic time points (B) were reverse transcribed. Oligonucleotides complementary to Gtsf1 were used to amplify the corresponding RNA sequences. (C) Western blot with affinity-purified anti-GTSF1 antibodies were used to detect a 29-kd band in postnatal day 3 ovaries and testis, but not liver. Fertility and Sterility 2009 91, 2020-2024DOI: (10.1016/j.fertnstert.2008.05.042) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A–D) GTSF1 expression in the ovaries. Immunohistochemistry was performed on newborn (A, B) and 8-week-old ovaries (C, D). GTSF1 can be clearly detected (B) in the cytoplasm of germ cell clusters (GCC) and primordial follicles (PF) in newborn ovaries. At 8 weeks, GTSF1 can be clearly seen in all stages of developing oocytes, from primordial (PF) and primary follicles (PrF) (D) to secondary (SF) and antral follicles (AF) (C). (E, F) GTSF1 expression in the testis. Immunohistochemistry was performed on postnatal day 10 testis. At postnatal day 10, GTSF1 expression can be seen in primary spermatogonia (SG) but not in primary spermatocytes (SC). Scale bars = 50 μm. Fertility and Sterility 2009 91, 2020-2024DOI: (10.1016/j.fertnstert.2008.05.042) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions