Clinical Nutrition Experimental

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Clinical Nutrition Experimental Therapeutic potential of probiotic Lactobacillus plantarum AD3 on acetaminophen induced uremia in experimental rats Arpita Patra, Shreya Mandal, Animesh Samanta, Keshab Chandra Mondal, Dilip K. Nandi Clinical Nutrition Experimental Volume 19, Pages 12-22 (June 2018) DOI: 10.1016/j.yclnex.2018.02.002 Copyright © 2018 The Authors Terms and Conditions

Fig. 1 Histological study of kidney tissues (at 20×). (A–D) Hematoxylin and eosin stained kidney sections: (A) Negative control rats (Gr. I), showing appearance of normal glomerulus (NG) and normal renal tubules (NT); (B) APAP exposed rats (Gr. II), showing multiple foci of hemorrhage, necrosis and cloudy swelling of tubules (DG for damaged glomerulus and DT for dilated tubules); (C) Kidney section of the rats co-treated with L. plantarum AD3 strain (Gr. III) showing almost normal appearance of with repaired glomerulus (RG) and repaired tubule (RT) in kidney and (D) probiotic control (Gr. IV) group showing normal histological appearance of kidney cell. Clinical Nutrition Experimental 2018 19, 12-22DOI: (10.1016/j.yclnex.2018.02.002) Copyright © 2018 The Authors Terms and Conditions

Fig. 2 DNA fragmentation assay. A. DNA isolated from experimental kidney tissues was loaded on 1% (w/v) agarose gel. Lane a and lane b: DNA isolated from Gr. III rat kidney samples; Lane c: DNA isolated from Gr. II rat kidney samples; Lane d: DNA isolated from Gr. I rat kidney samples. B. Quantitative measurement of DNA fragmentation. Clinical Nutrition Experimental 2018 19, 12-22DOI: (10.1016/j.yclnex.2018.02.002) Copyright © 2018 The Authors Terms and Conditions

Fig. 3 L. plantarum AD3 strain interaction with ileal mucosa fragments. Micrograph of the intestinal rat mucosa ex vivo, after 6 h of incubation, observed by scanning electron microscopy. Mucosa preserved on the ileum (A) epithelium non-infected. Infected tissue with L. plantarum AD3 showed bacteria (➞) adhered to the epithelium of the ileum (B). Clinical Nutrition Experimental 2018 19, 12-22DOI: (10.1016/j.yclnex.2018.02.002) Copyright © 2018 The Authors Terms and Conditions