The heart reinnervates after transplantation

Slides:

Advertisements

Similar presentations

The Cardiovascular System: The Heart

Advertisements

Digitized Cardiac Potentials Recorded with CR Leads

George E. Yanulis, MEng, MS, Pascal Lim, MD, Ali Ahmad, MD, Zoran B

Выполнил Финк Эдуард Студент 182 группы ОМ

Reentrant and Focal Activations During Atrial Fibrillation in Patients With Atrial Septal Defect Takashi Nitta, MD, PhD, Shun-ichiro Sakamoto, MD, PhD,

Mammalian Cardiac Regeneration After Fetal Myocardial Infarction Requires Cardiac Progenitor Cell Recruitment Myron Allukian, MD, Junwang Xu, PhD, Michael.

Dual-Chamber Pacing for Cardiomyopathies: A 1996 Clinical Perspective

Acidosis-induced apoptosis in human and porcine heart

Connexins and the atrioventricular node

Diastolic heart failure in anaesthesia and critical care

Ventricular and pulmonary vascular remodeling induced by pulmonary overflow in a chronic model of pretricuspid shunt Daniele Linardi, MD, Alessio Rungatscher,

An ovine model of postinfarction dilated cardiomyopathy

Mechanism of myocardial ischemia with an anomalous left coronary artery from the right sinus of Valsalva Carlo R. Bartoli, PhD, William B. Wead, PhD,

Robert H. Anderson, MD The Annals of Thoracic Surgery

Postsynaptic Levels of [Ca2+]i Needed to Trigger LTD and LTP

Linear end-systolic pressure-volume relationship during pulsatile left ventricular bypass represents native heart function Osamu Kawaguchi, MD, John.

Endothelial Expression of Bone Morphogenetic Protein Receptor Type 1a is Required for Atrioventricular Valve Formation Kan Kaneko, MD, PhD, Xiaodong.

Working heart model of heterotopic heart-lung transplantation in rats

E. Duco Jansen, Martin Frenz, Kamuran A Kadipasaoglu, T

George E. Yanulis, MEng, MS, Pascal Lim, MD, Ali Ahmad, MD, Zoran B

Nitric oxide–generating β-adrenergic blocker nipradilol preserves postischemic cardiac function Hitoshi Horimoto, MD, Adam E Saltman, MD, PhD, Glenn.

Cardiac Vagal Stimulation Eliminates Detrimental Tachycardia Effects of Dobutamine Used for Inotropic Support Youhua Zhang, MD, PhD, Todor N. Mazgalev,

Stem cell therapy in the aging hearts of Fisher 344 rats: Synergistic effects on myogenesis and angiogenesis Jiang-Yong Min, MD, Yu Chen, MD, Sohail.

Cytokines Link Toll-Like Receptor 4 Signaling to Cardiac Dysfunction After Global Myocardial Ischemia John Cha, MD, Zhiping Wang, MD, Lihua Ao, BS, Ning.

Radial approach: a new concept in surgical treatment for atrial fibrillation. II. Electrophysiologic effects and atrial contribution to ventricular filling

Volume 18, Issue 6, Pages (June 1997)

Intraoperative metabolic monitoring of the heart: II

Ablation of Ganglionic Plexi During Combined Surgery for Atrial Fibrillation Nicolas Doll, MD, PhD, Patrick Pritzwald-Stegmann, MD, Markus Czesla, MD,

Functional assessment of skeletal muscle ventricles after pumping for up to four years in circulation Gregory A Thomas, MD, Robert L Hammond, PhD, Kevin.

Myocardial tactile stiffness during acute reduction of coronary blood flow Kagami Miyaji, MD, Seiryo Sugiura, MD, Hirotaka Inaba, MD, Shinichi Takamoto,

In-Vivo Dynamic Deformation of the Mitral Valve Anterior Leaflet

Isolation of genes differentially expressed at the downstream anastomosis of prosthetic arterial grafts with use of mRNA differential display Allen D.

Role of apoptosis in myocardial stunning after open heart surgery

Regional distribution of atrial electrical changes induced by stimulation of extracardiac and intracardiac neural elements Pierre L. Pagé, MDa,b, Nazih.

Clifford H. Van Meter, MD (by invitation), William C

Pericardium-Lined Skeletal Muscle Ventricles: Up to Two Years' In-Circulation Experience1 Gregory A Thomas, Susumu Isoda, Robert L Hammond, Huiping Lu,

A dynamic and chamber-specific mitochondrial remodeling in right ventricular hypertrophy can be therapeutically targeted Jayan Nagendran, MD, Vikram.

Alejandro Aris, MD, PhD The Annals of Thoracic Surgery

Volume 13, Issue 1, Pages (January 2016)

Glucocorticoids reduce ischemia-reperfusion-induced myocardial apoptosis in immature hearts Jeffrey M Pearl, MD, David P Nelson, MD, PhD, Steven M Schwartz,

Marinus A. Borgdorff, Beatrijs Bartelds, Michael G

Ventricular morphology and coronary arterial anatomy in hearts with isomeric atrial appendages Hideki Uemura, MD, Siew Yen Ho, PhD, Robert H Anderson,

Efficacy of Thalamocortical and Intracortical Synaptic Connections

Takashi Nitta, MD, Masataka Mitsuno, MD, Chris K

Surgical ablation for atrial fibrillation: The efficacy of a novel bipolar pen device in the cardioplegically arrested and beating heart Shun-ichiro.

Load dependence of cardiac output in biventricular pacing: right ventricular pressure overload in pigs David G Rabkin, MD, Santos E Cabreriza, MBA, Lauren.

Hermann Aebert, MD, Torsten Cornelius, BS, Tobias Ehr, BS, Stephan R

Double-outlet right ventricle with complete atrioventricular canal

Modification of Infarct Material Properties Limits Adverse Ventricular Remodeling Masato Morita, MD, Chad E. Eckert, PhD, Kanji Matsuzaki, MD, PhD, Mio.

Thomas J. van Brakel, MD, Gil Bolotin, MD, PhD, Kenneth J

Brian A. Bruckner, MD, Limael E

Hemodynamic Assessment After Complete Repair of Pulmonary Atresia With Major Aortopulmonary Collaterals Richard D. Mainwaring, MD, V. Mohan Reddy, MD,

Protecting the aged heart during cardiac surgery: Use of del Nido cardioplegia provides superior functional recovery in isolated hearts Arun Govindapillai,

Victor Z Han, Kirsty Grant, Curtis C Bell Neuron

Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs Jiang-Yong.

Gregory A Thomas, MD, Larry W Stephenson, MD

Acute hemodynamic benefit of multisite ventricular pacing after congenital heart surgery Frank J Zimmerman, MD, Joanne P Starr, MD, Peter R Koenig, MD,

Hyper-response to cardiac resynchronization with permanent His bundle pacing: Is parahisian pacing sufficient? Olujimi A. Ajijola, MD, PhD, Jorge Romero,

George Berberian, MD, T. Alexander Quinn, MS, Joshua P

Late results of palliative atrial switch for transposition, ventricular septal defect, and pulmonary vascular obstructive disease Harold M Burkhart,

Effect of Surgical Reconstruction on Flow Profiles in the Aorta Using Magnetic Resonance Blood Tagging Mark A. Fogel, MD, Paul M. Weinberg, MD, Alison.

Cardiac Purkinje cells

Application of microwave energy in cardiac tissue ablation: from in vitro analyses to clinical use Mathew R Williams, MD, Michael Knaut, MD, Dany Bérubé,

Shirin Lalezari, MD, Edris A. F. Mahtab, PhD, Margot M

Evan Loh, MD, Edward B. Lankford, MD, PhD, David J

Effects of acute left ventricular unloading on right ventricular function in normal and chronic right ventricular pressure-overloaded lambs Boudewijn.

Skeletal muscle ventricle aortic counterpulsation: function during chronic heart failure Bhavik G Patel, MD, Sachin H Shah, MD, Lou I Astra, MD, Robert.

Epicardial atrial pacemaker lead placement after multiple cardiac operations John C Kucharczuk, MD, Mitchell I Cohen, MD, Larry A Rhodes, MD, Tom R Karl,

Alexandra B Nelson, Claudia M Krispel, Chris Sekirnjak, Sascha du Lac

James L. Cox, MD The Journal of Thoracic and Cardiovascular Surgery

Presentation transcript:

The heart reinnervates after transplantation David A Murphy, MD, Gregory W Thompson, BSc, Jeffrey L Ardell, PhD, Rollin McCraty, Robert S Stevenson, BS, Virgilio E Sangalang, MD, René Cardinal, PhD, Michael Wilkinson, PhD, Sylvia Craig, DVM, Frank M Smith, PhD, John G Kingma, PhD, J.Andrew Armour, MD, PhD The Annals of Thoracic Surgery Volume 69, Issue 6, Pages 1769-1781 (June 2000) DOI: 10.1016/S0003-4975(00)01240-6

Fig 1 Holter monitor electrocardiographic data obtained from (A, B) 2 conscious animals 1 year after cardiac acute transplantation. The upper and lower panels on the left illustrate data obtained during the slowest (A, 26 bpm; B, 31 bpm) and fastest (A, 163 bpm; B, 120 bpm) heart rates in each dog. To the right are power spectral data (PSD) derived from representative 20-minute periods when bradycardia occurred in both animals. These data display similar power spectral peaks despite the fact that the heart of the dog in A exhibited no evidence of parasympathetic reinnervation, whereas that of the dog in B was reinnervated by parasympathetic efferent neurons. The Annals of Thoracic Surgery 2000 69, 1769-1781DOI: (10.1016/S0003-4975(00)01240-6)

Fig 2 Varied cardiac effects elicited in the 2 dogs in Figure 1 (A and B represent the same animals) induced by stimulating efferent axons in sectioned right (RV) or left (LV) cervical vagus individually. The times when stimuli were applied to neural tissues are indicated by the solid black sections (stimulus artifacts) recorded in the electrocardiographic traces. Note that bradycardia was elicited in 1 animal (B) but not in the other (A). (ECG = lead II electrocardiogram; LVP = left ventricular chamber pressure.) The Annals of Thoracic Surgery 2000 69, 1769-1781DOI: (10.1016/S0003-4975(00)01240-6)

Fig 3 Gross and microscopic analyses of suture lines 1 year after transplantation. (A) Gross anatomy of suture line on intimal surface of ascending aorta. (B) Masson’s trichrome stain of suture line of aorta (Ao) and pulmonary artery (PA) obtained from tissue specimen in A. (C) Neurofilament staining of same tissue demonstrating a relatively large nerve coursing over suture line to cardiac tissue at bottom of Figure. (D) Enlargement of tissue in boxed area of C. Note the deficit where a suture was located (small arrow) and axons coursing through the suture line (large arrow). Calibration bars: B and C = 10 mm; D = 2 mm. The Annals of Thoracic Surgery 2000 69, 1769-1781DOI: (10.1016/S0003-4975(00)01240-6)

Fig 4 Cardiac effects elicited by stimulating decentralized (A) left or (B) right stellate ganglion individually in animal with substantial reinnervation. Heart rate increased during right stellate ganglion stimulation, and left ventricular systolic pressure rose when either stellate ganglion was stimulated. (ECG = lead II electrocardiogram; LV IMP = left ventricular intramyocardial pressure; LVP = left ventricular chamber pressure.) The Annals of Thoracic Surgery 2000 69, 1769-1781DOI: (10.1016/S0003-4975(00)01240-6)

Fig 5 Responses displayed by two inferior vena cava–inferior right atrial neurons to intracellular or connecting nerve stimulation in vitro. (A) An action potential (upper trace) was generated by a depolarizing current pulse (1-ms duration) (lower trace) delivered into a neuron by way of the intracellular recording electrode. (B) A synaptically generated action potential occurred in the same neuron after stimulating a nerve (arrow stim) connected to the ganglion containing the identified neuron (arrow) before (Pre Hex) but not after (Hex) hexamethonium administration. Vertical bars: A = 10 mV; B = 20 mV; horizontal bars: A and B = 10 ms. The Annals of Thoracic Surgery 2000 69, 1769-1781DOI: (10.1016/S0003-4975(00)01240-6)

Fig 6 Saturable specific binding of [3H]CGP12177 to tissues (2 mm in diameter, 350 μm thick) obtained from left ventricles of control (○) and transplanted (•) hearts. Combined data derived from the ventricles of 4 study dogs and 3 control dogs are shown. The Annals of Thoracic Surgery 2000 69, 1769-1781DOI: (10.1016/S0003-4975(00)01240-6)

Fig 7 (A) Northern blot analysis was performed using specific complementary DNA probes; 18S ribosomal RNA was used as an internal control. Expression levels of messenger RNA for both α-tubulin (α-Tub) and β-tubulin (β-Tub) were augmented in myocardial tissues obtained from two transplanted hearts (T4, T6) compared with levels in control heart tissue (C). Myocardial tissue mRNA was substantially degraded in tissues obtained from a third animal (ie, T3; blot not shown). (B) Immunoblot analysis of tissue from the same myocardium demonstrating either a substantial decrease (ie, T3, T4) or no change (ie, T6) in total tubulin levels compared with control myocardial tissue. Myosin heavy chain (MHC) from a corresponding Coomassie blue–stained gel is also shown. The Annals of Thoracic Surgery 2000 69, 1769-1781DOI: (10.1016/S0003-4975(00)01240-6)