Nitric oxide may inhibit neointimal hyperplasia by decreasing isopeptidase T levels and activity in the vasculature Nick D. Tsihlis, PhD, Muneera R.

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Presentation transcript:

Nitric oxide may inhibit neointimal hyperplasia by decreasing isopeptidase T levels and activity in the vasculature Nick D. Tsihlis, PhD, Muneera R. Kapadia, MD, Ashley K. Vavra, MD, Walker D. Flannery, BA, Christopher S. Oustwani, BA, Qun Jiang, MD, Melina R. Kibbe, MD Journal of Vascular Surgery Volume 58, Issue 1, Pages 179-186 (July 2013) DOI: 10.1016/j.jvs.2012.10.066 Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 1 Nitric oxide (NO) inhibits isopeptidase T activity. Isopeptidase T activity was assayed with and without S-nitroso-N-acetylpenicillamine (SNAP) (A) at 60 minutes and (B) every 5 minutes for 60 minutes. N-acetylpenicillamine (NAP, 1000 μmol/L) is a negative control. n = 3 per treatment group. Data are representative of four separate experiments. *P < .001 vs control, **P < .02 vs control. Journal of Vascular Surgery 2013 58, 179-186DOI: (10.1016/j.jvs.2012.10.066) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 2 Nitric oxide (NO)-mediated inhibition of isopeptidase T activity is prevented by reducing agents. Isopeptidase T activity was assessed with S-nitroso-N-acetylpenicillamine (SNAP, 1000 μmol/L) ± dithiothreitol (DTT, 5 mmol/L) or glutathione (GSH, 5 mmol/L). t = 60 minutes, n = 3 per each treatment group. Data are representative of four separate experiments. *P < .001 vs control, †P < .001 vs SNAP. Journal of Vascular Surgery 2013 58, 179-186DOI: (10.1016/j.jvs.2012.10.066) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 3 Nitric oxide (NO) causes S-nitrosylation of isopeptidase T (SNO-IsoT) in a time-dependent manner. After treatment of vascular smooth muscle cells (VSMCs) with S-nitroso-N-acetylpenicillamine (SNAP, 500 μmol/L) ± dithiothreitol (DTT, 5 mmol/L) for the times indicated, SNO-isopeptidase T was enriched from cell lysates via biotin switch assay, then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) and Western blot analysis. Data are representative of three separate experiments. Journal of Vascular Surgery 2013 58, 179-186DOI: (10.1016/j.jvs.2012.10.066) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 4 Nitric oxide (NO) does not affect isopeptidase T (isoT) levels or intracellular localization in vascular smooth muscle cells (VSMCs). A, Western blot analysis of isopeptidase T levels in VSMC exposed to S-nitroso-N-acetylpenicillamine (SNAP, 125-1000 μmol/L) for 24 hours showed no effect of NO. B, Immunofluorescence staining performed on VSMC for isopeptidase T (red) after exposure to SNAP (1000 μmol/L) for 24 hours showed mostly cytoplasmic staining and no change in levels or localization with NO. Blue represents nuclei stained with 4′,6-diamidino-2-phenylindole. Data and images are representative of at least two separate experiments. rIsoT, Recombinant isopeptidase T. Journal of Vascular Surgery 2013 58, 179-186DOI: (10.1016/j.jvs.2012.10.066) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 5 Nitric oxide (NO) decreases levels of isopeptidase T (isoT) and free ubiquitin in vivo, with a concomitant increase in ubiquitinated proteins. A, Immunofluorescence staining for isopeptidase T (red) performed on balloon-injured rat carotid arteries treated with and without 10 mg PROLI/NO for 3 or 14 days showed that isopeptidase T increased after injury and decreased with NO. Nuclei are shown in blue (4',6-diamidino-2-phenylindole), and internal elastic lamina autofluorescence is visible in green. The negative control (ie, no primary antibody) revealed no red staining. Data are representative of two separate stains. B, Western blot analysis performed on balloon-injured rat carotid artery lysates treated with and without 20 mg PROLI/NO for 3 days showed a significant decrease in isopeptidase T levels (left) in the injury + NO group. Free ubiquitin levels also decreased, with a concomitant increase in ubiquitinated proteins (right) observed in the injury + NO group. rIsoT, Recombinant isopeptidase T. *P < .03 vs control. n = 3/group. Journal of Vascular Surgery 2013 58, 179-186DOI: (10.1016/j.jvs.2012.10.066) Copyright © 2013 Society for Vascular Surgery Terms and Conditions