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The relative amounts of the different acid-base forms of alanine as pH varies

The relative amounts of the different acid-base forms of lysine as pH varies

Electrophoresis Separation of Amino acids using pI

Figure 19.2 Separation of a protein mixture by electrophoresis. At pH = 6.00, a neutral protein does not migrate, a basic protein is protonated and migrates toward the negative electrode, and an acidic protein is deprotonated and migrates toward the positive electrode. Fig. 19-2, p. 800

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