Fanconi anemia group J mutation abolishes its DNA repair function by uncoupling DNA translocation from helicase activity or disruption of protein-DNA complexes.

Slides:



Advertisements
Similar presentations
Volume 6, Issue 3, Pages (September 2000)
Advertisements

Analysis of human α globin gene mutations that impair binding to the α hemoglobin stabilizing protein by Xiang Yu, Todd L. Mollan, Andrew Butler, Andrew.
Patient-derived C-terminal mutation of FANCI causes protein mislocalization and reveals putative EDGE motif function in DNA repair by Luca Colnaghi, Mathew.
Volume 67, Issue 1, Pages e3 (July 2017)
The Molecular Basis for Cross-Reacting Material–Positive Hemophilia A Due to Missense Mutations Within the A2-Domain of Factor VIII by Kagehiro Amano,
Sebastian D Fugmann, David G Schatz  Molecular Cell 
Volume 17, Issue 1, Pages (January 2005)
Volume 6, Issue 3, Pages (September 2000)
Volume 22, Issue 3, Pages (May 2006)
Volume 135, Issue 1, Pages (July 2008)
Volume 6, Issue 3, Pages (September 2000)
The structure of the GPIb–filamin A complex
The interferon regulatory factor ICSBP/IRF-8 in combination with PU
Homologous Recombination Rescues Mismatch-Repair-Dependent Cytotoxicity of SN1- Type Methylating Agents in S. cerevisiae  Petr Cejka, Nina Mojas, Ludovic.
HES1 is a novel interactor of the Fanconi anemia core complex
RNAi in Human Cells Molecular Cell
Volume 1, Issue 5, Pages (June 2002)
Replication-Independent Histone Deposition by the HIR Complex and Asf1
RAG1/2-Mediated Resolution of Transposition Intermediates
UV-Induced RPA1 Acetylation Promotes Nucleotide Excision Repair
Division of Labor in an Oligomer of the DEAD-Box RNA Helicase Ded1p
Ben B. Hopkins, Tanya T. Paull  Cell 
Ivar Ilves, Tatjana Petojevic, James J. Pesavento, Michael R. Botchan 
Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes
A Rad51 Presynaptic Filament Is Sufficient to Capture Nucleosomal Homology during Recombinational Repair of a DNA Double-Strand Break  Manisha Sinha,
Stephen Schuck, Arne Stenlund  Molecular Cell 
Volume 22, Issue 2, Pages (February 2014)
BRCA1-Independent Ubiquitination of FANCD2
Beena Krishnan, Lila M. Gierasch  Chemistry & Biology 
Volume 23, Issue 6, Pages (September 2006)
Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis  Beth A. Rousseau, Zhonggang Hou, Max J. Gramelspacher,
Single-Molecule Analysis Reveals Differential Effect of ssDNA-Binding Proteins on DNA Translocation by XPD Helicase  Masayoshi Honda, Jeehae Park, Robert.
Volume 16, Issue 1, Pages (June 2016)
Volume 21, Issue 7, Pages (November 2017)
Volume 8, Issue 5, Pages (November 2001)
Volume 35, Issue 1, Pages (July 2009)
Volume 59, Issue 6, Pages (September 2015)
Progressive Structural Transitions within Mu Transpositional Complexes
Volume 10, Issue 5, Pages (November 2002)
Volume 25, Issue 21, Pages (November 2015)
Volume 21, Issue 2, Pages (October 2017)
Claudia Schneider, James T. Anderson, David Tollervey  Molecular Cell 
Pierre-Henri L Gaillard, Eishi Noguchi, Paul Shanahan, Paul Russell 
Volume 28, Issue 3, Pages (November 2007)
Volume 90, Issue 4, Pages (August 1997)
DNA-Induced Switch from Independent to Sequential dTTP Hydrolysis in the Bacteriophage T7 DNA Helicase  Donald J. Crampton, Sourav Mukherjee, Charles.
Volume 59, Issue 4, Pages (August 2015)
Diverse Pore Loops of the AAA+ ClpX Machine Mediate Unassisted and Adaptor- Dependent Recognition of ssrA-Tagged Substrates  Andreas Martin, Tania A. Baker,
Volume 8, Issue 5, Pages (November 2001)
Replication-Independent Histone Deposition by the HIR Complex and Asf1
Amanda Solem, Nora Zingler, Anna Marie Pyle  Molecular Cell 
Exon Identity Established through Differential Antagonism between Exonic Splicing Silencer-Bound hnRNP A1 and Enhancer-Bound SR Proteins  Jun Zhu, Akila.
Volume 54, Issue 6, Pages (June 2014)
Volume 29, Issue 2, Pages (February 2008)
Volume 117, Issue 1, Pages (April 2004)
Volume 10, Issue 15, Pages (August 2000)
Excision of the Drosophila Mariner Transposon Mos1
Volume 56, Issue 3, Pages (November 2014)
Volume 95, Issue 5, Pages (November 1998)
Daniel L. Kaplan, Mike O'Donnell  Molecular Cell 
Volume 4, Issue 5, Pages (November 1999)
Transcriptional Regulation by p53 through Intrinsic DNA/Chromatin Binding and Site- Directed Cofactor Recruitment  Joaquin M Espinosa, Beverly M Emerson 
Michael J. McIlwraith, Stephen C. West  Molecular Cell 
Volume 105, Issue 7, Pages (June 2001)
Volume 35, Issue 1, Pages (July 2009)
Volume 7, Issue 6, Pages (June 2001)
H3K4me3 Stimulates the V(D)J RAG Complex for Both Nicking and Hairpinning in trans in Addition to Tethering in cis: Implications for Translocations  Noriko.
Volume 13, Issue 1, Pages (October 2015)
Volume 28, Issue 4, Pages (November 2007)
Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA
Presentation transcript:

Fanconi anemia group J mutation abolishes its DNA repair function by uncoupling DNA translocation from helicase activity or disruption of protein-DNA complexes by Yuliang Wu, Joshua A. Sommers, Avvaru N. Suhasini, Thomas Leonard, Julianna S. Deakyne, Alexander V. Mazin, Kazuo Shin-ya, Hiroyuki Kitao, and Robert M. Brosh Blood Volume 116(19):3780-3791 November 11, 2010 ©2010 by American Society of Hematology

Purification and determination of iron content in FANCJ-WT and FANCJ-A349P proteins. Purification and determination of iron content in FANCJ-WT and FANCJ-A349P proteins. (A) Cartoon depicting FANCJ protein with the conserved helicase core domain and position of the conserved Fe-S domain. The conserved helicase motifs are indicated by yellow boxes, and the BRCA1 binding domain is indicated by green. The Fe-S domain is expanded, and the 4 conserved cysteines are indicated with blue, with the A349P missense mutation of a FANCJ patient marked. (B) The purity of the FANCJ-WT and FANCJ-A349P proteins was evaluated by their detected migration after sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on Coomassie-stained gels according to their predicted sizes. For each protein, 2 μg was loaded. (C) Stoichiometry of iron atoms per purified recombinant FANCJ-WT or FANCJ-A349P. The determination of iron content is described in supplemental Methods. Data represent the mean of at least 3 independent experiments, with SD indicated by error bars. Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology

FANCJ-A349P mutant is inactive as a helicase on forked duplex and G-quadruplex DNA substrates that FANCJ-WT efficiently unwinds. FANCJ-A349P mutant is inactive as a helicase on forked duplex and G-quadruplex DNA substrates that FANCJ-WT efficiently unwinds. (A) Helicase reactions (20 μL) were performed by incubating the indicated FANCJ concentration with a 0.5nM forked duplex DNA substrate at 30°C for 15 minutes as described in supplemental Methods. Triangle, heat-denatured DNA substrate control. (B) Helicase reactions were performed the same as with panel A, except that G4 DNA was used instead of forked duplex. M, radiolabeled TP-G4 49-mer oligonucleotide (supplemental Table 1) marker. Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology

DNA binding of mutant and wild-type FANCJ proteins as detected by gel mobility shift assays. DNA binding of mutant and wild-type FANCJ proteins as detected by gel mobility shift assays. (A) The indicated concentrations of FANCJ-WT or FANCJ-A349P protein were incubated with 0.5nM forked duplex DNA substrate at 25°C for 30 minutes, as described in supplemental Methods. The DNA-protein complexes were resolved on native 5% polyacrylamide gels. (B) Quantitative analyses of DNA gel-shift experiments as performed in (A). (C) Gel mobility shift experiments with radiolabeled single-stranded 67-mer oligonucleotide. (D) Quantitative analyses of DNA gel-shift experiments as performed in (C). (E) 2.4nM FANCJ-WT or FANCJ-A349P was incubated with 0.5nM radiolabeled forked duplex DNA at 25°C for 15 minutes, and the indicated concentration of 67-mer ssDNA was subsequently added and incubated for an additional 15 minutes at 25°C. DNA-protein complexes were resolved on native 5% polyacrylamide gels. Quantitative analyses of DNA-binding data from DNA competition experiments are shown with SD indicated by error bars. Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology

FANCJ-A349P translocates on single-stranded DNA FANCJ-A349P translocates on single-stranded DNA. The Keff-ssDNA values (A) and kcat values (B) for ATP hydrolysis catalyzed by FANCJ-WT and FANCJ-A349P proteins were determined as a function of oligonucleotide dT length, as described in supplemental Methods. FANCJ-A349P translocates on single-stranded DNA. The Keff-ssDNA values (A) and kcat values (B) for ATP hydrolysis catalyzed by FANCJ-WT and FANCJ-A349P proteins were determined as a function of oligonucleotide dT length, as described in supplemental Methods. Error bars represent the SD of the fit to the Michaelis-Menton equation. (C) Cartoon depicting fluorescence quenching of fluorescein covalently attached to 3′ end of ssDNA molecule by ATP-driven translocation of FANCJ to a position near the fluorophore. Binding site size or the assembly state of FANCJ is not known. For simplicity, cartoon depicts initial ATP-dependent translocation of FANCJ followed by second translocation step placing FANCJ in the vicinity of fluorescein where it quenches the fluorophore; however, physical or kinetic step size is not known. (D) Fluorescence quenching experiments, performed as described in “Fluorescence quench translocation assays,” with use of FANCJ-WT, FANCJ-A349P, and FANCJ-K52R proteins. Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology

FANCJ-A349P is compromised in its ability to displace DNA-bound protein. FANCJ-A349P is compromised in its ability to displace DNA-bound protein. (A) The indicated concentrations of FANCJ-WT (upper) or FANCJ-A349P (lower) were incubated with 2mM ATP and DNA substrate (0.5nM) that had streptavidin bound to the covalently linked biotin moiety residing 52 nt from the 5′ end of the radiolabeled oligonucleotide (supplemental Table 1). (B) Quantitative analyses of FANCJ streptavidin displacement assays as shown in panel A. Data represent the mean of at least 3 independent experiments with SD indicated by error bars. (C) Experimental scheme to examine FANCJ displacement of RAD51 protein filament on ssDNA. (D) The degradation of the intact 32P-labeled ssDNA fragment by exonuclease VII was analyzed in a 10% polyacrylamide gel. The preformed RAD51-ssDNA complex was incubated with FANCJ-WT (lanes 4-7) or FANCJ-A349P (lanes 9-12) followed by the addition of exonuclease VII. Protein concentrations are indicated at the top of the gel. The ssDNA fragment before and after the treatment with exonuclease VII is shown in lanes 1 and 2, respectively. (E) The data from panel D represented as a graph. Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology

Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology

Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology

Expression of FANCJ-A349P fails to rescue sensitivity of FA-J cells to the G-quadruplex binding compound telomestatin and exerts a dominant-negative effect on telomestatin resistance of wild-type cells. Expression of FANCJ-A349P fails to rescue sensitivity of FA-J cells to the G-quadruplex binding compound telomestatin and exerts a dominant-negative effect on telomestatin resistance of wild-type cells. (A) TMS sensitivity of stably transfected human FA-J (EUFA30/hTert/SV40) fibroblasts with the indicated genotypes was evaluated by survival assay. (B) γ-H2AX immunofluorescence staining of the corresponding FA-J cell lines stably transfected with plasmids encoding GFP, GFP-FANCJ-WT, or GFP-FANCJ-A349P. FA-J cells were untreated or treated with 5μM TMS for 4 hours, washed with PBS, allowed to recover for 4 hours, and analyzed by immunofluorescence detection. (C) Quantitative analyses of γ-H2AX foci after 5μM TMS exposure of the corresponding transfected FA-J cell lines shown in panel B. Data represent the mean of at least 100 cells counted, with SD indicated by error bars. (D) TMS sensitivity of stably transfected human wild-type (VH10/hTert) fibroblasts with the indicated genotypes was evaluated by survival assay. (E) γ-H2AX immunofluorescence staining of wild-type cells transfected with plasmids encoding GFP, GFP-tagged FANCJ-WT, or GFP-tagged FANCJ-A349P. Wild-type cells were either untreated or exposed to 5μM TMS for 4 hours, washed with PBS, allowed to recover for 4 hours, and analyzed by immunofluorescence detection. (F) Quantitative analyses of γ-H2AX foci in the corresponding transfected wild-type cell lines as shown in panel E. Yuliang Wu et al. Blood 2010;116:3780-3791 ©2010 by American Society of Hematology