Jinous Samavat, M. Sc. , Ilaria Natali, Ph. D

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Presentation transcript:

Acrosome reaction is impaired in spermatozoa of obese men: a preliminary study Jinous Samavat, M.Sc., Ilaria Natali, Ph.D., Selene Degl'Innocenti, M.Sc., Erminio Filimberti, M.Sc., Giulia Cantini, Ph.D., Alessandra Di Franco, M.Sc., Giovanna Danza, Ph.D., Giuseppe Seghieri, M.D., Marcello Lucchese, M.D., Elisabetta Baldi, Ph.D., Gianni Forti, M.D., Michaela Luconi, Ph.D. Fertility and Sterility Volume 102, Issue 5, Pages 1274-1281.e2 (November 2014) DOI: 10.1016/j.fertnstert.2014.07.1248 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Spermatozoa of obese men display higher levels of Sp-AR and reduced ARPC. Acrosome reaction was evaluated with the fluorescent probe lectin in swim-up selected and capacitated spermatozoa of obese (n = 13) and lean (n = 19) patients in basal conditions (Sp-AR) or after stimulation with P (P-AR). The Sp-AR, P-AR, and ARPC data are reported as box charts, indicating statistical significance (P) as evaluated by Student's t test for unpaired samples. P<.0001. Fertility and Sterility 2014 102, 1274-1281.e2DOI: (10.1016/j.fertnstert.2014.07.1248) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Cholesterol content in spermatozoa is higher in obese than in lean men. Total cholesterol was evaluated in 10 μg of NaOH-extracted spermatozoa from obese (n = 23) and lean (n = 21) subjects by GS/MS (A). Data are reported as box charts, indicating statistical significance (P) as evaluated by Student's t test for unpaired samples. Representative filipin staining (red) in spermatozoa from an obese (B) and a lean (C) subject. Corresponding microphotographs (D, E) in bright-field (×40 magnification). Filipin staining is concentrated in sperm head, with a faint signal in sperm tails. P<.05. Fertility and Sterility 2014 102, 1274-1281.e2DOI: (10.1016/j.fertnstert.2014.07.1248) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Correlations of anthropometric parameters and E2 with Sp-AR and ARPC in obese and lean subjects. Correlations of BMI, weight, waist, and E2 with Sp-AR (A, C, E, G) or ARPC (B, D, F, H) are shown. Pearson's analysis for normally distributed data. Age-adjusted r, P, and n values of a multivariate analysis are indicated. Fertility and Sterility 2014 102, 1274-1281.e2DOI: (10.1016/j.fertnstert.2014.07.1248) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions