Volume 16, Issue 10, Pages (October 2009)

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Volume 16, Issue 10, Pages 1031-1044 (October 2009) Deciphering Biosynthesis of the RNA Polymerase Inhibitor Streptolydigin and Generation of Glycosylated Derivatives Carlos Olano, Cristina Gómez, María Pérez, Martina Palomino, Antonio Pineda-Lucena, Rodrigo J. Carbajo, Alfredo F. Braña, Carmen Méndez, José A. Salas Chemistry & Biology Volume 16, Issue 10, Pages 1031-1044 (October 2009) DOI: 10.1016/j.chembiol.2009.09.015 Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 1 Structures of Streptolydigin, Streptolydigin Aglycon, and Novel Glycosylated Derivatives Chemistry & Biology 2009 16, 1031-1044DOI: (10.1016/j.chembiol.2009.09.015) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 2 Genetic Organization of the Streptolydigin Gene Cluster and UPLC Analysis of S. lydicus Strains (A) Genetic organization of the streptolydigin gene cluster. White arrows indicate genes not involved in streptolydigin biosynthesis. Black and gray arrows indicate genes involved in streptolydigin biosynthesis. Gray arrows indicate genes deleted in mutant SLM7H13 and disrupted in mutants SLMX27, SLM4C1, and SLM961. Slg6E5, Slg4A8, Slg9C7, and Slg6G6 represent cosmids containing the sequenced region. Asterisks show the BamHI fragments used for disruption in mutants SLM4C1 and SLM961. BamHI sites are numbered. B: BglII, M: MfeI, N: NruI P: PstI and S: StuI sites used for cloning purposes. (B) UPLC analysis of S. lydicus wild-type (WT) and mutant SLM961, SLM4C1, and SLMX27. AU: arbitrary units. 1: Streptolydigin. Chemistry & Biology 2009 16, 1031-1044DOI: (10.1016/j.chembiol.2009.09.015) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 3 Proposed Pathway for Streptolydigin Biosynthesis In bold, PKS domains that differ from the predicted model. LD: loading module, M: module, KS: ketosynthase, AT: acyl transferase, a: malonyl-CoA specific, p: methylmalonyl-CoA specific, ACP: acyl carrier protein, DH: dehydratase, KR: ketoreductase, C: condensation, A: adenylation, PCP: peptidyl carrier protein. Chemistry & Biology 2009 16, 1031-1044DOI: (10.1016/j.chembiol.2009.09.015) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 4 UPLC Analysis of S. lydicus Strains UPLC analysis of S. lydicus strain SLM7H13 (A) and expressing pEM4T8-12 (B), pFL844T (C), pFL845T (D), and pLNBIVT (E). AU: arbitrary units. Chemistry & Biology 2009 16, 1031-1044DOI: (10.1016/j.chembiol.2009.09.015) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 5 Bioactivity Testing of Streptolydigin (1), Streptolydiginone (3), and Streptolydigin LA (4) against S. albus Each paper disk was soaked with 2 μg of the corresponding compound dissolved in 15 μl methanol. Negative control (C) contained 15 μl methanol and no antibiotic. Chemistry & Biology 2009 16, 1031-1044DOI: (10.1016/j.chembiol.2009.09.015) Copyright © 2009 Elsevier Ltd Terms and Conditions