Mass spectrometry analysis.

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Biochemistry Part 3 Lehningers Biochemistry. Amino Acid.

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Mass spectrometry analysis. Mass spectrometry analysis. Non‐denaturing ESI‐MS was used to characterize RXR‐α–TBT complexes in (A) the absence or in (B,C) the presence of a fourfold molar excess of TIF2 peptide. Increasing ion acceleration voltage (Vc) from 50 V (A,B) to 190 V (C) had no effect on the interaction of TBT with RXR. LC‐MS analysis of RXR‐α–TBT complexes either (D) purified or (E) solubilized from crystals was carried out to assess the reversibility of TBT binding. Measured mass corresponds to unbound RXR‐α LBD. ESI‐MS, electrospray ionization mass spectrometry; LBD, ligand‐binding domain; LC‐MS, liquid chromatography‐mass spectrometry; RXR‐α, retinoid X receptor‐α; TBT, tributyltin; TIF2, transcriptional intermediary factor 2. Albane le Maire et al. EMBO Rep. 2009;10:367-373 © as stated in the article, figure or figure legend