Analysis of CDCP1 as a potential prostate cancer biomarker.

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Analysis of CDCP1 as a potential prostate cancer biomarker. Analysis of CDCP1 as a potential prostate cancer biomarker. Microvesicles were isolated from PC-3 and RWPE-1 cells as indicated under “Experimental Procedures.” The amounts of protein loaded per lane are indicated in the figure. CDCP1 (A) and calreticulin (B) were then detected in lysates (Lys) and microvesicles (Ves) by Western blot. Bands at the predicted molecular weight for CDCP1 (140 kDa) and calreticulin (63 kDa) were observed. C, Microvesicles were isolated from conditioned media of PC-3 cells and LNCaP as indicated under “Experimental Procedures.” Sample buffer was added and samples were loaded on SDS-PAGE gels. The presence of CDCP1 in the samples was analyzed by Western blot. D, Microvesicles were isolated from the conditioned media of PC-3 cells and immunoisolated by EpCAM-dynabeads. Bound microvesicles were lyzed in sample buffer and loaded on SDS-PAGE gels. The presence of CDCP1 and caveolin-1 was analyzed by Western blot. Bands at the predicted molecular weight for CDCP1 (140 kDa) and caveolin-1 (22 kDa) were observed. The figure shows representative gels of 2–4 experiments. Kirsten Sandvig, and Alicia Llorente Mol Cell Proteomics 2012;11:M111.012914 © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.