Adeno-associated Virus Serotype Vectors Efficiently Transduce Normal Prostate Tissue and Prostate Cancer Cells Jianzhong Ai, Dan Wang, Qiang Wei, Hong.

Slides:

Advertisements

Similar presentations

Volume 49, Issue 1, Pages (January 2006)

Advertisements

Genetic Manipulation of Brown Fat Via Oral Administration of an Engineered Recombinant Adeno-associated Viral Serotype Vector Wei Huang, Travis McMurphy,

The Origin of the Bone Scan as a Tumour Marker in Prostate Cancer

Figure S1 a b c d DAPI Lgr β-catenin Merge

Knockdown of Bone Morphogenetic Proteins Type 1a Receptor (BMPR1a) in Breast Cancer Cells Protects Bone from Breast Cancer-Induced Osteolysis by Suppressing.

Volume 49, Issue 1, Pages (January 2006)

Targeted Disruption of the Lama3 Gene in Adult Mice Is Sufficient to Induce Skin Inflammation and Fibrosis Monika Pesch, Sabrina König, Monique Aumailley

Volume 49, Issue 1, Pages (January 2006)

RNAscope The Journal of Molecular Diagnostics

Local gene delivery of heme oxygenase-1 by adeno-associated virus into osteoarthritic mouse joints exhibiting synovial oxidative stress S. Kyostio-Moore,

STAU1/2 and YBX1 recruit poly-GR/PR into large cytoplasmic granules.

A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene Expression in AAV-Mediated Gene Transfer Grit Taschenberger,

Volume 16, Issue 12, Pages (December 2008)

Is Benign Prostatic Hyperplasia (BPH) an Immune Inflammatory Disease?

Volume 24, Issue 2, Pages (February 2016)

Molecular Therapy - Methods & Clinical Development

The Origin of the Bone Scan as a Tumour Marker in Prostate Cancer

Volume 61, Issue 1, Pages (January 2012)

Blood-derived smooth muscle cells as a target for gene delivery

Volume 6, Issue 5, Pages (May 2016)

Long-Term Rescue of a Lethal Inherited Disease by Adeno-Associated Virus–Mediated Gene Transfer in a Mouse Model of Molybdenum-Cofactor Deficiency S.

G.-I. Im, H.-J. Kim Osteoarthritis and Cartilage

Volume 10, Issue 1, Pages (January 2018)

Radiation resistance and stem-like cells in brain tumors

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Volume 18, Issue 10, Pages (October 2010)

Tissue-engineered, hydrogel-based endothelial progenitor cell therapy robustly revascularizes ischemic myocardium and preserves ventricular function

Molecular Therapy - Methods & Clinical Development

Volume 15, Issue 11, Pages (November 2007)

Volume 24, Issue 2, Pages (July 2018)

Volume 23, Issue 12, Pages (December 2015)

Absence of Distinguishing Senescence Traits in Human Melanocytic Nevi

A Recombinant Baculovirus Efficiently Generates Recombinant Adeno-Associated Virus Vectors in Cultured Insect Cells and Larvae Yang Wu, Liangyu Jiang,

Volume 20, Issue 1, Pages (January 2012)

Volume 10, Issue 2, Pages (February 2018)

Volume 113, Issue 10, Pages (November 2017)

Volume 18, Issue 4, Pages (April 2010)

Volume 5, Issue 2, Pages (February 2002)

Collagen VII Half-Life at the Dermal-Epidermal Junction Zone: Implications for Mechanisms and Therapy of Genodermatoses Tobias Kühl, Markus Mezger, Ingrid.

Molecular Therapy - Methods & Clinical Development

Volume 21, Issue 4, Pages (April 2013)

Airway smooth muscle remodeling is a dynamic process in severe long-standing asthma Muhannad Hassan, MD, Taisuke Jo, MD, PhD, Paul-André Risse, PhD,

Molecular Therapy - Methods & Clinical Development

T.M. Alie, P.J. Vrljicak, D.B. Myburgh, I.R. Gupta

Rita U. Lukacs, Sanaz Memarzadeh, Hong Wu, Owen N. Witte

Volume 18, Issue 1, Pages (January 2010)

LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes, and reducing RhoB abundance impairs the.

Alexandra V. Lucs, Allan L. Abramson, Bettie M. Steinberg

Fig. 3 CSF1 is expressed in human melanoma.

Volume 10, Issue 4, Pages (October 2004)

Organization of Stem Cells and Their Progeny in Human Epidermis

Volume 13, Issue 3, Pages (March 2006)

Volume 23, Issue 12, Pages (December 2015)

Volume 18, Issue 3, Pages (March 2010)

Correlation of reovirus RNA/protein with proliferating tumor cells

Molecular Therapy - Nucleic Acids

Volume 6, Issue 3, Pages (September 2002)

Volume 34, Issue 4, Pages (August 2015)

Volume 154, Issue 1, Pages (July 2019)

Volume 23, Issue 3, Pages (March 2015)

Volume 24, Issue 1, Pages (January 2016)

Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.

The BRCA1 aggregates exclude large nuclear structures.

Neuroprotection by Gene Therapy Targeting Mutant SOD1 in Individual Pools of Motor Neurons Does not Translate Into Therapeutic Benefit in fALS Mice Chris.

Volume 24, Issue 2, Pages (February 2016)

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

Fetal Brain-directed AAV Gene Therapy Results in Rapid, Robust, and Persistent Transduction of Mouse Choroid Plexus Epithelia Marie Reine Haddad, Anthony.

Molecular Therapy - Methods & Clinical Development

Volume 22, Issue 7, Pages (July 2014)

Molecular Therapy - Methods & Clinical Development

Presentation transcript:

Adeno-associated Virus Serotype Vectors Efficiently Transduce Normal Prostate Tissue and Prostate Cancer Cells Jianzhong Ai, Dan Wang, Qiang Wei, Hong Li, Guangping Gao European Urology Volume 69, Issue 1, Pages 179-181 (January 2016) DOI: 10.1016/j.eururo.2015.10.019 Copyright © 2015 Terms and Conditions

Fig. 1 rAAV6.2 and rAAV7 efficiently transduced mouse prostate following intraprostate injection. (A) Quantification of transduction efficiency in AP (yellow bars) and DLP (orange bars) following intraprostate injection with rAAV vectors of different serotypes expressing EGFP. EGFP fluorescence intensity of cryosections is presented in arbitrary units (a.u.) (B) Representative fluorescence images of AP and DLP cryosections showing merger of EGFP native fluorescence (green) and nuclear staining by DAPI (blue) following injection of PBS, rAAV6.2, or rAAV7. Scale bars, 25μm. (C) Biodistribution of rAAV genomes in AP (yellow bars) and DLP (orange bars) following intraprostate injection of rAAV6.2 and rAAV7. Data are presented as rAAV genome copies per diploid genome. (D) Representative images of immunofluorescence staining of prostate luminal cells (top panels), basal cells (middle panels), and stromal cells (bottom panels), marked by K8, K5, and α-actin staining, respectively. Native EGFP fluorescence images and merged images with DAPI staining (blue) for the same sections are also shown. Arrows indicate representative co-localization of EGFP and cell-type marker signals. (E) Quantification of the percentage of EGFP-positive cells for each cell type. rAAV = recombinant adeno-associated virus; AP=anterior prostate; DLP=dorsal lateral prostate; EGFP=enhanced green fluorescent protein; DAPI=4′,6-diamidino-2-phenylindole. European Urology 2016 69, 179-181DOI: (10.1016/j.eururo.2015.10.019) Copyright © 2015 Terms and Conditions