S100A12 provokes mast cell activation: A potential amplification pathway in asthma and innate immunity Zheng Yang, PhD, Wei Xing Yan, PhD, Hong Cai,

Slides:

Advertisements

Similar presentations

Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components Stephen R. Reeves, MD, PhD, Tessa.

Advertisements

The K+ channel iKCA1 potentiates Ca2+ influx and degranulation in human lung mast cells S Mark Duffy, PhD, Patrick Berger, MD, PhD, Glenn Cruse, Weidong.

Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma Kyung S. Lee, MS, Sun M. Jin,

Mechanisms of vitamin D3 metabolite repression of IgE-dependent mast cell activation Kwok-Ho Yip, PhD, Natasha Kolesnikoff, PhD, Chunping Yu, BSc(Hons),

Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI Tanya M. Laidlaw, MD, John W. Steinke,

Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M

Weiguo Chen, MD, PhD, Yasuhiro Tabata, MD, PhD, Aaron M

Liping Xie, MD, John T. Schroeder, PhD, Jacqueline M

TNF-α–mediated bronchial barrier disruption and regulation by src-family kinase activation Michelle A. Hardyman, PhD, Emily Wilkinson, BSc, Emma Martin,

Ryan H. Dougherty, MD, Sukhvinder S

Umasundari Sivaprasad, PhD, David J. Askew, PhD, Mark B

The sphingosine-1-phosphate/sphingosine-1-phosphate receptor 2 axis regulates early airway T-cell infiltration in murine mast cell–dependent acute allergic.

Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids Kittipong Maneechotesuwan, MD, PhD, Sirinya.

Increased TGF-β2 in severe asthma with eosinophilia

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation Yuki Nakamura,

Ramipril and metoprolol intake aggravate human and murine anaphylaxis: Evidence for direct mast cell priming Maria Nassiri, MS, Magda Babina, PhD, Sabine.

Allergen-dependent solubilization of IL-13 receptor α2 reveals a novel mechanism to regulate allergy Michael O. Daines, MD, Weiguo Chen, PhD, Yasuhiro.

FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells Shigeru Okumura, DDS, PhD, Hironori Sagara,

Autocrine hemokinin-1 functions as an endogenous adjuvant for IgE-mediated mast cell inflammatory responses Tina L. Sumpter, PhD, Chin H. Ho, MD, Anna.

Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation Si-Yen Liu, PhD, Wei-Zhi Wang,

Katherine J. Baines, PhD, BBiomedSci (Hons), Jodie L

Is 9 more than 2 also in allergic airway inflammation?

Dendritic cells and alveolar macrophages mediate IL-13–induced airway inflammation and chemokine production Margaret Crapster-Pregont, BS, Janice Yeo,

Curcumin, a constituent of curry, suppresses IgE-mediated allergic response and mast cell activation at the level of Syk Jun Ho Lee, PhD, Jie Wan Kim,

Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria Daisuke Fujisawa, MD, Jun-ichi Kashiwakura,

The anti-inflammatory effect of glucocorticoids is mediated by glucocorticoid-induced leucine zipper in epithelial cells Jane Eddleston, PhD, Jack Herschbach,

Pharmacologic inhibition of Notch signaling suppresses food antigen–induced mucosal mast cell hyperplasia Asuka Honjo, MD, Nobuhiro Nakano, PhD, Susumu.

Silvana Balzar, MD, Matthew Strand, PhD, Diane Rhodes, PhD, Sally E

Toll-like receptor 9 suppression in plasmacytoid dendritic cells after IgE-dependent activation is mediated by autocrine TNF-α John T. Schroeder, PhD,

IL-13 desensitizes β2-adrenergic receptors in human airway epithelial cells through a 15-lipoxygenase/G protein receptor kinase 2 mechanism Giusy D.

Toll-like receptor 3 enhances late-phase reaction of experimental allergic conjunctivitis Mayumi Ueta, MD, PhD, Satoshi Uematsu, MD, PhD, Shizuo Akira,

A specific sphingosine kinase 1 inhibitor attenuates airway hyperresponsiveness and inflammation in a mast cell–dependent murine model of allergic asthma

Critical role of IgE-dependent mast cell activation in a murine model of allergic conjunctivitis Ken Fukuda, MD, PhD, Masaharu Ohbayashi, PhD, Kei Morohoshi,

Oral immunotherapy induces local protective mechanisms in the gastrointestinal mucosa Stephanie A. Leonard, MD, Gustavo Martos, PhD, Wei Wang, MD, Anna.

Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent Brittany M. Salter, BSc, John Paul Oliveria,

Single–tree nut immunotherapy attenuates allergic reactions in mice with hypersensitivity to multiple tree nuts Mike Kulis, PhD, Yifan Li, BS, Hannah.

Jia Guo, MD, Xin Lin, PhD, Marc A

IL-13 dampens human airway epithelial innate immunity through induction of IL-1 receptor–associated kinase M Qun Wu, MD, PhD, Di Jiang, BS, Sean Smith,

MicroRNA-9 regulates steroid-resistant airway hyperresponsiveness by reducing protein phosphatase 2A activity Jing Jing Li, MS, Hock L. Tay, PhD, Steven.

Effects of budesonide and formoterol on allergen-induced airway responses, inflammation, and airway remodeling in asthma Margaret M. Kelly, MB, PhD,

Sphingosine-1-phosphate receptor 2 protects against anaphylactic shock through suppression of endothelial nitric oxide synthase in mice Hong Cui, MD,

Estrogen increases the severity of anaphylaxis in female mice through enhanced endothelial nitric oxide synthase expression and nitric oxide production

Nonreceptor tyrosine kinases ITK and BTK negatively regulate mast cell proinflammatory responses to lipopolysaccharide Weishan Huang, PhD, J. Luis Morales,

Activin A is an acute allergen-responsive cytokine and provides a link to TGF-β– mediated airway remodeling in asthma Christian Karagiannidis, MD, Gabriele.

Disruption of T-cell immunoglobulin and mucin domain molecule (TIM)–1/TIM4 interaction as a therapeutic strategy in a dendritic cell–induced peanut allergy.

Peanut-induced intestinal allergy is mediated through a mast cell–IgE–FcεRI–IL-13 pathway Meiqin Wang, MD, PhD, Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus Julie.

Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis Deepti R. Nagarkar, PhD, Julie A. Poposki,

Src homology 2 domain–containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung Sun-Young Oh, PhD,

Cysteinyl leukotriene upregulates IL-11 expression in allergic airway disease of mice Kyung Sun Lee, PhD, So Ri Kim, MD, PhD, Hee Sun Park, MD, PhD, Seoung.

Oral administration of a synthetic agonist of Toll-like receptor 9 potently modulates peanut-induced allergy in mice Fu-Gang Zhu, PhD, Ekambar R. Kandimalla,

Lisa G. Wood, PhD, Manohar L. Garg, PhD, Peter G. Gibson, MBBS

CC chemokine receptors CCR1 and CCR4 are expressed on airway mast cells in allergic asthma Kawa Amin, PhD, Christer Janson, MD, PhD, Ilkka Harvima, MD,

Connective tissue growth factor expression is regulated by histamine in lung fibroblasts: Potential role of histamine in airway remodeling Steffen Kunzmann,

Novel allergic asthma model demonstrates ST2-dependent dendritic cell targeting by cypress pollen Lucia Gabriele, BS, Giovanna Schiavoni, BS, Fabrizio.

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

H4 histamine receptor mediates optimal migration of mast cell precursors to CXCL12 Veronique Godot, PhD, Michel Arock, MD, Gilles Garcia, MD, Francis.

IL-13 dampens human airway epithelial innate immunity through induction of IL-1 receptor–associated kinase M Qun Wu, MD, PhD, Di Jiang, BS, Sean Smith,

Activation of human mast cells through the platelet-activating factor receptor Naoki Kajiwara, PhD, Tomomi Sasaki, BSc, Peter Bradding, DM, Glenn Cruse,

Mechanisms of allergen-specific desensitization

Prostaglandin E receptor subtype EP3 in conjunctival epithelium regulates late-phase reaction of experimental allergic conjunctivitis Mayumi Ueta, MD,

The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells Zuyi Weng, MS, PhD, Arti B. Patel, MS, Smaro Panagiotidou, MA, Theoharis.

Zhenying Nie, PhD, Cole S. Nelson, PhD, David B. Jacoby, MD, Allison D

Mice deficient in the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation Takumi Kiwamoto,

The extra domain A of fibronectin is essential for allergen-induced airway fibrosis and hyperresponsiveness in mice Martin Kohan, MSc, Andres F. Muro,

Prostaglandin E2–EP3 signaling suppresses skin inflammation in murine contact hypersensitivity Tetsuya Honda, MD, PhD, Toshiyuki Matsuoka, MD, PhD, Mayumi.

Mice deficient in the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation Takumi Kiwamoto,

Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids Kittipong Maneechotesuwan, MD, PhD, Sirinya.

Aarti Shikotra, BSc, David F. Choy, BSc, Chandra M

TNF can contribute to multiple features of ovalbumin-induced allergic inflammation of the airways in mice Susumu Nakae, PhD, Carolina Lunderius, PhD,

Presentation transcript:

S100A12 provokes mast cell activation: A potential amplification pathway in asthma and innate immunity Zheng Yang, PhD, Wei Xing Yan, PhD, Hong Cai, PhD, Nicodemus Tedla, PhD, Chris Armishaw, PhD, Nick Di Girolamo, PhD, Hong Wei Wang, PhD, Taline Hampartzoumian, PhD, Jodie L. Simpson, PhD, Peter G. Gibson, John Hunt, PhD, Prue Hart, PhD, J. Margaret Hughes, PhD, Michael A. Perry, PhD, Paul F. Alewood, PhD, Carolyn L. Geczy, PhD Journal of Allergy and Clinical Immunology Volume 119, Issue 1, Pages 106-114 (January 2007) DOI: 10.1016/j.jaci.2006.08.021 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, S100A12 provoked histamine (HT) release from human lung MCs. Lung tissue was incubated with S100A12 for 45 minutes (open bars) or 90 minutes (solid bars). Duplicates from tissues from 2 donors are given. B and C, IgE-primed CBMCs were incubated with S100A12 1 hour before challenge with control rabbit IgG (open bars) or anti-IgE (0.07 μg/mL, solid bars), IgE–anti-IgE without S100A12, or control IgG (gray bars). Fig 1, B, shows histamine (HT) levels from 4 donors, and Fig 1, C, shows LTC4 levels from 3 donors. ∗P < .05 and ∗∗P < .01 compared with IgE–anti-IgE; #P < .05 and ##P < .01 compared with media control. Journal of Allergy and Clinical Immunology 2007 119, 106-114DOI: (10.1016/j.jaci.2006.08.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 MCs colocalize in human asthmatic airways/bronchial tissue. A, S100A12-positive leukocytes in the submucosal region (a) were in close proximity to tryptase-positive MCs (b) in adjacent serial sections. (c) No S100A12-positive cells were evident in apparently normal lung tissue. B, S100A12-positive eosinophils were prominent within a mucus plug in asthmatic airways. Adjacent sections show EP-positive (red) and S100A12-positive (brown) eosinophils (arrows). Scale bar = 20 μm. C, S100A12 levels in sputum from subjects with eosinophilic asthma were higher than those in healthy subjects (P < .0001) and subjects with other asthma subtypes (P = .0086). Journal of Allergy and Clinical Immunology 2007 119, 106-114DOI: (10.1016/j.jaci.2006.08.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 S100A12 activates MCs in vivo.A, Footpads (FP) injected with S100A12 (hatched bars) or vehicle (open bars) are shown; cromoglycate pretreatment (solid bars) reduced edema. ∗P < .0005 compared with vehicle; #P < .005 compared with mice treated with S100A12 alone. Inset shows S100A12-induced plasma leakage in the “blue” right foot compared with the left foot (control). B, S100A12 did not induce edema in Wf/Wf mice. Footpad injection with vehicle (Wf/Wf mice, gray bars; WT mice, open bars) or S100A12 (Wf/Wf mice, solid bars; WT mice, hatched bars). #P < .005 compared with vehicle and with Wf/Wf mice injected with S100A12. C, Wf/Wf mice reconstituted with BMMCs from WT mice (solid bars) responded to S100A12 compared with vehicle (hatched bars), whereas no edema was seen in Wf/Wf mice in response to S100A12 (gray bars) or vehicle (open bars). ##P < .005 compared with unreconstituted mice. Journal of Allergy and Clinical Immunology 2007 119, 106-114DOI: (10.1016/j.jaci.2006.08.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 S100A12 provokes MC-dependent leukocyte transmigration in the mesenteric circulation. Superfusion of S100A12 (3.6 × 10−8 mol/L) or compound 48/80 (1.3 μmol/L) significantly increased leukocyte flux (A), adhesion (B), and extravasation (C), as assessed by means of intravital microscopy. Cromoglycate negated responses. Results are means ± SD from groups of 5 rats. ∗P < .001 compared with buffer control; #P < .01 compared with S100A12 alone. Journal of Allergy and Clinical Immunology 2007 119, 106-114DOI: (10.1016/j.jaci.2006.08.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 A, Histamine release provoked by S100A12 was partially suppressed by 1–10 μmol/L RAGE42-59. ∗P = .03 compared with S100A12. B, RAGE mRNA was not detected in CBMCs (lane 1); THP-1 cells (lane 2) expressed the gene. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA is shown. C, Western blotting for RAGE in lysates of HL60 cells (lane 1), THP-1 cells (lane 2), and CBMCs from 2 donors (lanes 3 and 4). Molecular weight markers are indicated. Journal of Allergy and Clinical Immunology 2007 119, 106-114DOI: (10.1016/j.jaci.2006.08.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions