Expression of neurotrophin 3 and its tropomyosin-related kinase receptor C in human preantral follicles Galia Oron, M.D., Asangla Ao, Ph.D., Or Friedman,

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Presentation transcript:

Expression of neurotrophin 3 and its tropomyosin-related kinase receptor C in human preantral follicles Galia Oron, M.D., Asangla Ao, Ph.D., Or Friedman, B.Sc., Benjamin Fisch, M.D., Ph.D., Xiao Yun Zhang, M.Sc., Avi Ben-Haroush, M.D., M.M.Sc., Yoav Peled, M.D., Ronit Abir, Ph.D. Fertility and Sterility Volume 95, Issue 6, Pages 2056-2062 (May 2011) DOI: 10.1016/j.fertnstert.2011.02.012 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Immunohistochemistry photographs of NT3 protein expression. (A) Section of human ovary from a 31-gestational-week-old fetus. Note the primordial follicles, the weak red-brown staining indicating NT3 expression in oocytes (full cytoplasmic and nuclear staining), and staining in the GCs and a portion of the stroma cells. Original magnification ×400. (B) Section of human ovary from a 22-year-old woman. Note the primordial follicles with the red-brown staining indicating NT3 protein expression in the oocytes (full cytoplasmic staining and nuclear staining) and in the GCs and a portion of the stroma cells. Original magnification ×400. (C) Positive control for NT3 protein expression of section of mouse skin. Note the red-brown staining in the sample. Original magnification ×400. (D) Negative control for the same ovarian section as in A. Note the primordial follicles with the overall blue staining and the lack of red-brown staining. Original magnification ×400. (E) Negative control for the same ovarian section as in B. Note the primordial follicles with the overall blue staining and the lack of red-brown staining. Original magnification ×400. Fertility and Sterility 2011 95, 2056-2062DOI: (10.1016/j.fertnstert.2011.02.012) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Immunohistochemistry photographs of TrkC protein expression and ISH photographs of TrkC mRNA expression. (A) Immunohistochemistry section of human ovary from a 33-gestational-week-old fetus. Note the primordial follicles with red-brown staining indicating TrkC protein expression in the oocytes (full cytoplasmic staining and nuclear staining in some of the oocytes) and in the GCs and in a portion of the stroma cells. Original magnification ×400. (B) In situ hybridization section of human ovary from a 17-year-old girl. Note the primordial-primary follicle with weak mRNA oocyte staining (partial cytoplasmic staining without nuclear staining), with mRNA staining in the GCs and stroma cells. Original magnification ×400. (C) Negative immunohistochemistry control for the same ovarian section as in A. Note the primordial follicles, the overall blue staining, and the lack of red-brown staining. Original magnification ×400. (D) Negative ISH control for the same ovarian section as in B. Note the primordial follicles with the overall blue staining and the lack of red-brown staining. Original magnification ×400. (E) Positive ISH control for α-tubulin in the same ovarian section as in A and C. Note the primordial follicles, with the red-brown staining indicating expression in the oocytes (full cytoplasmic and nuclear staining) and in the GCs and stroma cells. Original magnification ×400. Fertility and Sterility 2011 95, 2056-2062DOI: (10.1016/j.fertnstert.2011.02.012) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Representative RT-PCR gel illustrating TrkC gene and NT3 gene expression in fetal and adult ovaries. (A) TrkC isoform I and II (392 bp and 350 bp, respectively). (B) TrkC isoform III (374 bp). (C) NT3 isoform I (309 bp). (D) NT3 isoform II (363 bp). (E) β-Actin (274 bp). Lane 1, 100-bp DNA ladder. Lane 2, Ovary from a 21-year-old woman. Lane 3, Ovary from a 29-year-old woman. Lane 4, Ovary from a 39-year-old woman. Lane 5, Ovary from a 21-gestational-week-old fetus. Lane 6, Ovary from a 22-gestational-week-old fetus. Lane 7, Ovary from a 25-gestational-week-old fetus. Lane 8, Ovary from a 27-gestational-week-old fetus. Lane 9, RT− control. Fertility and Sterility 2011 95, 2056-2062DOI: (10.1016/j.fertnstert.2011.02.012) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions