Anibal A Acosta, M. D. , Laura Elberger, M. D. , Mario Borghi, M. D

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Presentation transcript:

Endometrial dating and determination of the window of implantation in healthy fertile women Anibal A Acosta, M.D., Laura Elberger, M.D., Mario Borghi, M.D., Juan C Calamera, Ph.D., Hector Chemes, M.D., Gustavo F Doncel, M.D., Ph.D., Harvey Kliman, Ph.D., Baltasar Lema, M.D., Livia Lustig, M.D., Sergio Papier, M.D. Fertility and Sterility Volume 73, Issue 4, Pages 788-798 (April 2000) DOI: 10.1016/S0015-0282(99)00605-6

Figure 1 Surface of an endometrial biopsy specimen during the luteal phase (day 25). Cells with microvilli (MV) cover most of the surface. Ciliated cells (C) are interspaced between them. Pinopodes (P) are seen among the other cell types. Notice that some pinopodes show a few remaining microvilli in their surface (∗). Original magnification, ×8,800. Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)

Figure 2 Pinopodes at higher magnification. In different areas the remains of preexisting microvilli can be observed (→). Original magnification, ×20,000. Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)

Figure 3 Graphic representation of the characteristics and expression of pinopodes during the luteal phase (the two half panels in each day represent the two biopsies from two different volunteers). Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)

Figure 4 Top: A cluster of pinopodes surrounds the opening of an endometrial gland (∗). There are numerous cells with microvilli, some of them with budding structures (B) probably indicating the initial stages of pinopode formation (luteal phase, day 20, original magnification, ×5,000). Bottom: The surface of the endometrium is extensively covered by pinopodes. Cells with microvilli are very rare (same patient, luteal phase, day 27, original magnification, ×2,500). Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)

Figure 5 Immunohistochemistry of β3 (A–C) and α4 (D–F) integrin subunits in cryostat sections of endometrial biopsy specimens at different days of the luteal phase. No expression of β3 integrin subunit was detected at day 20 (A), whereas a strong reaction was observed in the glandular (B) and luminal epithelium (C) at day 24. No expression of α4 integrin subunit was detected in the glandular and luminal epithelium at day 15 (D), whereas a strong reaction was observed in the glandular epithelium at day 22 (E) and at day 23 (F). Original magnification, ×150 (A, B, D, and E); ×300 (C and F). Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)

Figure 6 Graphic representation of integrins expression (αvβ3 and α4β1) in superficial and glandular epithelium and in the stroma during the luteal phase. “Sup. epith.” = superficial epithelium; “gl. epith.” = glandular epithelium. Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)

Figure 7 Graphic representation of leukemia inhibitory (LIF) and IL-1R tI expression during the luteal phase. “Sup. epith.” = superficial epithelium; “gl. epith.” = glandular epithelium. Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)

Figure 8 Graphic representation of MAG, MUC-1, and PrR expression in superficial and glandular epithelium during the luteal phase (note the blood groups of the patients for MAG determinations, in the top panel row). “Sup. epith.” = superficial epithelium; “gl. epith.” = glandular epithelium. Acosta. Window of implantation. Fertil Steril 2000. Fertility and Sterility 2000 73, 788-798DOI: (10.1016/S0015-0282(99)00605-6)