Volume 49, Issue 1, Pages 3-5 (January 2013) Spp1 Links Sites of Meiotic DNA Double-Strand Breaks to Chromosome Axes  Bernard de Massy  Molecular Cell  Volume 49, Issue 1, Pages 3-5 (January 2013) DOI: 10.1016/j.molcel.2012.12.011 Copyright © 2013 Elsevier Inc. Terms and Conditions

Figure 1 Connecting Meiotic DSB Sites to Chromosome Axes Most of the Spo11 accessible sites in S. cerevisiae are nucleosome depleted regions (NDR) upstream of transcription start sites (TSSs). The Set1 complex interacting with RNA Pol II modifies the +1 nucleosome and promotes H3K4me3 (green star). The Spp1 protein, which is localized to the chromosome axis through an interaction with Mer2, is able to bind H3K4me3 (arrow). This promotes the tethering of the chromatin loop to the axis and Spo11 interaction with the Rec114/Mei4/Mer2 complex. The association of the RMM complex to the axes depends on the proteins Red1 and Hop1 and is influenced by the cohesin Rec8. Molecular Cell 2013 49, 3-5DOI: (10.1016/j.molcel.2012.12.011) Copyright © 2013 Elsevier Inc. Terms and Conditions