Single-cell characterization of ovarian spheroids David Morse, PhD student Harvard University, Weitz Lab University of Cambridge, Knowles Lab

Ovarian cancer spheroids

Single-cell sequencing Bulk Sequencing Microfluidic-enabled single-cell profiling Single-cell Sequencing

Bulk Sequencing Single-cell Sequencing

Radial-spatial resolution Fluorescence Cell count Fluorescent distribution within a spheroid 200 µm

A pipeline for drug development Enable the design of effective drug cocktails and combination therapies Establish a method for examining context dependent single-cell gene expression cultured spheres were resistant to many common chemotherapeutics, most notably proteasome inhibitors, but were highly sensitive to the pro-oxidant elesclomol broad downregulation of genes involved in cell cycle signaling