Zinc fingers poke zebrafish, cause thrombosis!

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Zinc fingers poke zebrafish, cause thrombosis! by Pudur Jagadeeswaran Blood Volume 124(1):9-10 July 3, 2014 ©2014 by American Society of Hematology

Schematic diagram showing mechanism of antithrombin knockout in zebrafish. Schematic diagram showing mechanism of antithrombin knockout in zebrafish. The wild-type zebrafish and at3 knockout zebrafish are shown on top of their respective columns. Note the blood spot showing DIC in knockout fish. The straight bars represent at3. Zinc fingers are portrayed by the ovals with 3 fingers; Fok1 domains are represented by the green rectangles. The DNA-binding domains (not labeled) are located where the 3 fingers touch at3. DNA cleavage is portrayed by the gap in at3; NHEJ is denoted by the cluster of vertical lines shown within at3. A portion of the coagulation cascade (consisting of clotting factors VII, X, and their activated forms VIIa and Xa) initiated by TF and leading to the conversion of prothrombin to thrombin is shown on the left side. Thrombin and Xa are inhibited by antithrombin. The “X” depicts the lack of production of antithrombin. Pudur Jagadeeswaran Blood 2014;124:9-10 ©2014 by American Society of Hematology