Lgr5-Positive Cells are Cancer-Stem-Cell-Like Cells in Gastric Cancer

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Lgr5-Positive Cells are Cancer-Stem-Cell-Like Cells in Gastric Cancer Cell Physiol Biochem 2015;36:2447-2455 - DOI:10.1159/000430205 Fig. 1. CRCs express high Lgr5 in some cancer cells. (A-B) Lgr5 levels were examined in 50 resected GC specimens, compared with the paired adjacent normal tissue (NT) from the same patient, by RT-qPCR (A), and by Western blot (B). (C) Representative images for Lgr5 in GC and NT. (D) Tumor sphere formation. (E) A representative image for Lgr5 in tumor-sphere-forming GC cells. *p<0.05. n=50. Statistics: one-way ANOVA with a Bonferoni correction, followed by Fisher's Exact Test to compare two groups. Scale bar is 50µm. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

Lgr5-Positive Cells are Cancer-Stem-Cell-Like Cells in Gastric Cancer Cell Physiol Biochem 2015;36:2447-2455 - DOI:10.1159/000430205 Fig. 2. AAV-pLgr5-DTA specifically ablates Lgr5-positive GC cells. (A) Schematic of an AAV-pLgr5-DTA. The transduction with this virus specifically kills Lgr5-positive cells. AAV-pLgr5-null was also prepared, and used as a control for viral transduction. (B-C) Twelve hours after GC cells were transduced with viruses, the apoptosis of the cells was evaluated, showing by representative images (B), and by quantification (C). (D) Forty-eight hours after viral transduction, the GC cells were harvested for RT-qPCR for Lgr5. *p<0.05. n=5. Statistics: one-way ANOVA with a Bonferoni correction, followed by Fisher's Exact Test to compare two groups. Scale bar is 20µm. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

Lgr5-Positive Cells are Cancer-Stem-Cell-Like Cells in Gastric Cancer Cell Physiol Biochem 2015;36:2447-2455 - DOI:10.1159/000430205 Fig. 3. Ablation of Lgr5-positive cells inhibits growth of GCs in vitro. Cell growth was quantified in a MTT assay. *p<0.05. n=5. Statistics: one-way ANOVA with a Bonferoni correction, followed by Fisher's Exact Test to compare two groups. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

Lgr5-Positive Cells are Cancer-Stem-Cell-Like Cells in Gastric Cancer Cell Physiol Biochem 2015;36:2447-2455 - DOI:10.1159/000430205 © 2015 S. Karger AG, Basel - CC BY-NC 3.0