C D Coomassie stained gel PD:RNF4 KDa KDa 170 130 116 95 97 72 66 55

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C D Coomassie stained gel PD:RNF4 KDa KDa 170 130 116 95 97 72 66 55 43 45 34 29 26 Ponceau stained nitrocellulose membrane PD: RNF4 KDa 170 130 95 72 55 66 43 34 26 E F PD:RNF4 PD:RNF4 72 72 Ponceau stained nitrocellulose membrane Ponceau stained nitrocellulose membrane KDa KDa 170 170 130 130 95 95 72 72 55 55 43 43 34 34 26 26 Fig. 1 Raw data. The complete western blots are shown and the areas taken for inclusion in the panels in Fig. 1 are highlighted (indicated by boxes).

A RNF4(input) IB:RNF4 72 Ponceau stained nitrocellulose membrane KDa 170 130 95 72 55 43 34 C RNF4(input) PD:RNF4 RNF4(input) PD:RNF4 Ponceau stained nitrocellulose membrane Ponceau stained nitrocellulose membrane KDa KDa 170 130 170 130 95 95 72 72 55 55 43 43 34 34 Fig. 2A and C. Raw data. The complete western blots are shown and the areas taken for inclusion in the panels in Fig. 2 are highlighted (indicated by boxes).

B Left RNF4(input) PD:RNF4 72 72 Ponceau stained nitrocellulose membrane KDa 170 130 95 72 55 43 34 B Right RNF4(input) PD:RNF4 72 72 Ponceau stained nitrocellulose membrane KDa 170 130 95 72 55 43 34 Fig. 2B. Raw data. The complete western blots are shown and the areas taken for inclusion in the panels in Fig. 2 are highlighted (indicated by boxes).