Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis ShriHari S. Kadkol, Annette Bruno, Carol Dodge,

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Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis ShriHari S. Kadkol, Annette Bruno, Carol Dodge, Valerie Lindgren, Farhad Ravandi The Journal of Molecular Diagnostics Volume 6, Issue 1, Pages 22-27 (February 2004) DOI: 10.1016/S1525-1578(10)60487-4 Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Primer locations in relation to known breakpoints in the CBFβ and MYH11 cDNA. The Journal of Molecular Diagnostics 2004 6, 22-27DOI: (10.1016/S1525-1578(10)60487-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Algorithm for the comprehensive analysis of CBFβ-MYH11 fusion transcripts. The Journal of Molecular Diagnostics 2004 6, 22-27DOI: (10.1016/S1525-1578(10)60487-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 First-round RT-PCR with primers 1/2 followed by nested PCR with primers 3/4 (A), 3/11 (B) or 3/12 (C). M-1Kb Plus molecular weight marker (Invitrogen). Lane 1, patient 1; lane 2, patient 2; lane 3, ME-1f2 cells; lane 4, HL-60; lane 5, water control. The Journal of Molecular Diagnostics 2004 6, 22-27DOI: (10.1016/S1525-1578(10)60487-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 First-round RT-PCR with primers 5/6 followed by nested PCR with primers 7/8 (A) or 7/9 (B). M-1Kb Plus molecular weight marker (Invitrogen). A: Lane 1, patient 1; lane 2, patient 2; lane 3, ME-1f2 cells; lane 4, HL-60; lane 5, water control. B: Lane 1, patient 1; lane 2, patient 2; lane 3, ME-1f2 cells; lane 4, water. The Journal of Molecular Diagnostics 2004 6, 22-27DOI: (10.1016/S1525-1578(10)60487-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 5 Effect of RNA degradation on amplification. A: Analysis with primers 1/2 followed by primers 3/4: lanes 1 and 2, two previous RNA samples from patient 1; lane 3, most recent sample from patient 1 (same as in lane 1, Figure 3A); lane 4, ME-1f2 cells; lane 5, HL-60; lanes 6 and 7, water controls. B: Analysis with primers 5/6 followed by primers 7/8. Lanes 1 and 2, two previous samples from patient 1; lane 3, most recent sample from patient 1 (same as lane 1, Figure 4A); lane 4, water control. The Journal of Molecular Diagnostics 2004 6, 22-27DOI: (10.1016/S1525-1578(10)60487-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions