Preparation and characterization of injectable fibrillar type I collagen and evaluation for pseudoaneurysm treatment in a pig model Paul J. Geutjes,

Slides:

Advertisements

Similar presentations

Assessment of mouse hind limb endothelial function by measuring femoral artery blood flow responses Chao-Hung Wang, MD, Kuo-Ti Chen, MS, Hsiu-Fu Mei,

Advertisements

Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular.

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold

Prevention of paraplegia during thoracic aortic cross-clamping: Importance of patent internal mammary arteries Joseph S. Giglia, MD, Gerald B. Zelenock,

Antithrombotic effect of grape seed proanthocyanidins extract in a rat model of deep vein thrombosis Yunjian Zhang, PhD, MD, Hanping Shi, PhD, MD, Wenjian.

Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type Stuart K. Williams,

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture Bryan W. Tillman, MD, PhD,

A case of intravascular epithelioid hemangioendothelioma occurring 14 years after coil embolization for an extracranial internal carotid artery aneurysm

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Decellularized vein as a potential scaffold for vascular tissue engineering Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.

In vivo PEG modification of vascular surfaces for targeted delivery

Inflammatory responses involving tumor necrosis factor receptor-associated factor 6 contribute to in-stent lesion formation in a stent implantation model.

Implantation of amniotic membrane as a vascular substitute in the external jugular vein of juvenile sheep Habibollah Peirovi, MD, Navid Rezvani, MD,

Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis April E. Nedeau, MD,

Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution Matthew Waltham, MA, PhD, Kevin Burnand, MS,

Chun-Yu Wong, MD, Margreet R. de Vries, BSc, Yang Wang, MD, Joost R

Intraluminal abdominal aortic aneurysm thrombus is associated with disruption of wall integrity Dave Koole, MD, Herman J.A. Zandvoort, MD, Arjan Schoneveld,

Gale Tang, MD, David N. Charo, Rong Wang, PhD, Israel F

Plaque in superficial femoral arteries indicates generalized atherosclerosis and vulnerability to coronary death: An autopsy study Søren Dalager, MD,

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model Jianping Dai, MD, PhD, Liliane.

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Rapid recurrence of cystic adventitial disease in femoral artery and an etiologic consideration for the cyst Keiji Oi, MD, PhD, Tetsuya Yoshida, MD,

Extrinsic compression of the external iliac artery following internal fixation of an acetabular fracture Erin Koelling, MD, Dipankar Mukherjee, MD Journal.

Robert A. Brenes, MD, Caroline C

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold

Ex vivo proof-of-concept of end-to-end scaffold-enhanced laser-assisted vascular anastomosis of porcine arteries Dara R. Pabittei, MD, PhD, Michal Heger,

Antithrombotic effect of grape seed proanthocyanidins extract in a rat model of deep vein thrombosis Yunjian Zhang, PhD, MD, Hanping Shi, PhD, MD, Wenjian.

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Decellularized vein as a potential scaffold for vascular tissue engineering Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.

Doxycycline inhibition of aneurysmal degeneration in an elastase-induced rat model of abdominal aortic aneurysm: Preservation of aortic elastin associated.

Avulsion injury to the profunda femoris artery after total hip arthroplasty Sandra Huynh, Ahmed Kayssi, MD, MSc, Kevin Koo, MD, Dheeraj Rajan, MD, Oleg.

Menno H. Raber, MD, Robbert Meerwaldt, MD, PhD, Rob J. van Det, MD

Attenuation of early atherosclerotic lesions by immunotolerance with β2 glycoprotein I and the immunomodulatory effectors interleukin 2 and 10 in a murine.

Percutaneous transplantation of genetically-modified autologous fibroblasts in the rabbit femoral artery: a novel approach for cardiovascular gene therapy

Nanoparticle-mediated endothelial cell-selective delivery of pitavastatin induces functional collateral arteries (therapeutic arteriogenesis) in a rabbit.

Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution Matthew Waltham, MA, PhD, Kevin Burnand, MS,

Duplex ultrasound changes in the great saphenous vein after endosaphenous laser occlusion with 808-nm wavelength Leonardo Corcos, MD, Sergio Dini, MD,

Paclitaxel coating of the luminal surface of hemodialysis grafts with effective suppression of neointimal hyperplasia Insu Baek, MS, Cheng Zhe Bai, PhD,

Mechanisms of arterial graft failure. II

Reendothelialization of isolated segments of the canine carotid artery with reference to the possible role of the adventitial vasa vasorum Qun Shi, MD,

Reduction of myointimal hyperplasia after arterial anastomosis by local injection of transforming growth factor β3 Jonathan Ghosh, MD, MRCS, Mohammed.

Recanalization of arterial thrombus, and inhibition with β-radiation in a new murine carotid occlusion model: mRNA expression of angiopoietins, metalloproteinases,

Therapeutic site selection is important for the successful development of collateral vessels Ayako Nishiyama, MD, PhD, Hiroyuki Koyama, MD, PhD, Tetsuro.

In vivo suppression of vein graft disease by nonviral, electroporation-mediated, gene transfer of tissue inhibitor of metalloproteinase-1 linked to the.

Successful treatment of an iatrogenic right hepatic artery pseudoaneurysm and stenosis with a stent graft Jared R. Hylton, MS, William C. Pevec, MD

Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular.

Randall Adolph, BS, David A. Vorp, PhD, David L. Steed, MD, Marshall W

Adventitial endothelial implants reduce matrix metalloproteinase-2 expression and increase luminal diameter in porcine arteriovenous grafts Helen M.

Therapeutic application of contrast-enhanced ultrasound and low-dose urokinase for thrombolysis in a porcine model of acute peripheral arterial occlusion

Hiromichi Miwa, MD, Takehisa Matsuda, PhD Journal of Vascular Surgery

Assessment of mouse hind limb endothelial function by measuring femoral artery blood flow responses Chao-Hung Wang, MD, Kuo-Ti Chen, MS, Hsiu-Fu Mei,

Treatment of iatrogenic femoral artery pseudoaneurysm with percutaneous thrombin injection Chiau-Suong Liau, MD, Feng-Ming Ho, MD, Ming-Fong Chen, MD,

Effects of heparin, desmopressin, and isovolemic hemodilution with dextran on thrombus formation in synthetic vessel grafts inserted into the vena cava.

Aortic Customize: A new alternative endovascular approach to aortic aneurysm repair using injectable biocompatible elastomer. An in vitro study Willem-Maarten.

Transluminal stent graft repair with Wallgraft endoprosthesis in a porcine arteriovenous graft pseudoaneurysm model Peter H. Lin, MD, Chris K. Johnson,

Gene expression changes evoked in a venous segment exposed to arterial flow Deborah Abeles, MD, Stephanie Kwei, MD, George Stavrakis, MS, Yuzhi Zhang,

Comparison of the vascular responses to balloon-expandable stenting in the coronary and peripheral circulations: Long-term results in an animal model.

Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia Masatake Katsu, MD, Hiroyuki.

A case of intravascular epithelioid hemangioendothelioma occurring 14 years after coil embolization for an extracranial internal carotid artery aneurysm

Reply Journal of Vascular Surgery

Femoral pseudoaneurysms after percutaneous access

Functional analysis of cryopreserved veins

Tuberculous pseudoaneurysm of the femoral artery complicated by cutaneous tuberculosis of the foot: A case report Kathryn Leccese, BSc, José Ferreira,

Endovascular repair of an acute blunt popliteal artery injury

Short-term dexamethasone treatment inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden transgenic mice Abbey Schepers, MD, Nuno M.M. Pires,

William C. Quist, MD, PhD, Frank W. LoGerfo, MD

Prospective randomized study evaluating an absorbable cyanoacrylate for use in vascular reconstructions Alan B. Lumsden, MD, Eugene R. Heyman, PhD Journal.

Gene expression changes evoked in a venous segment exposed to arterial flow Deborah Abeles, MD, Stephanie Kwei, MD, George Stavrakis, MS, Yuzhi Zhang,

Presentation transcript:

Preparation and characterization of injectable fibrillar type I collagen and evaluation for pseudoaneurysm treatment in a pig model Paul J. Geutjes, MSc, J. Adam van der Vliet, MD, PhD, Kaeuis A. Faraj, PhD, Noes de Vries, MPharm, Herman T.B. van Moerkerk, BSc, Ronnie G. Wismans, MSc, Thijs Hendriks, PhD, Willeke F. Daamen, PhD, Toin H. van Kuppevelt, PhD Journal of Vascular Surgery Volume 52, Issue 5, Pages 1330-1338 (November 2010) DOI: 10.1016/j.jvs.2010.05.118 Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 1 Morphology and length of purified type I collagen fibrils. Scanning and transmission electron micrograph of purified type I collagen fibrils (A) and (B), respectively, and the distribution of fibril length (μm) in the collagen preparation (C). Results are mean ± SD of three separate experiments. Bar in (A) is 1 μm and inset is 0.2 μm. Bar in (B) is 0.2 μm. Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 2 Effect of sterilization procedure on the integrity of collagen. SDS-PAGE under reducing conditions of fibrillar type I collagen before (lane 1) and after (lanes 2-4) sterilization, viz.: ethylene oxide gas sterilization (lane 2), hydrogen peroxide (H2O2) gas sterilization (lane 3), or gamma irradiation (lane 4). The low molecular weight marker is visualized in lane 5. This is a combined image taken from two separate gels (lanes 1, 2, and 4 are from one gel). Note that especially after gamma sterilization, collagen degradation products are observed. Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 3 Effect of sterilization on platelet aggregation of fibrillar collagen. Collagen before sterilization was set on 1 arbitrary unit (AU). Results are mean ± SD for three separate experiments. Sterilized collagen showed no statistical significant differences in comparison to nonsterilized collagen (P > .05). EtO, Ethylene oxide gas sterilization; Gamma, gamma irradiation; H2O2, hydrogen peroxide sterilization. Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 4 Evaluation of injectability. Macroscopic image of the displacement test set up with type I collagen suspension in a 1 mL syringe (A). Graph of displacement test (1.8 mm/s with maximal distance 44 mm) of 3%, 4%, and 5% (w/v) type I collagen suspension in a 1 mL syringe with a 2F (0.7 mm) needle (B). On the Y-axis, force is given in Newton, and on the X-axis displacement is shown in mm. Results are mean ± SD of three separate experiments. The white arrow and white dashed line indicate the 1 mL syringe in the displacement set up. The black arrow and dashed circle indicate the pressure plate. Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 5 Aggregation of injectable collagen under physiologic conditions. Macroscopic images of 4% (w/v) type I collagen suspension in MilliQ water (A) and the network that was formed directly after injection of the type I collagen in pre-heated (37°C) phosphate buffered saline (PBS, pH 7.4) (B). Scanning electron microscopic images after applying a 0.5% (w/v) collagen suspension in MilliQ water or in PBS, resulting in an open fibril network (C) and an aggregated fibril network (D), respectively. Under physiologic conditions (PBS, 37°C), the collagen fibrils formed a dense network. Arrows in (A) and (B) indicate the aggregated type I collagen suspension. Arrowheads for (D) indicate the aggregated collagen fibrils. Bar is 10 μm in (C) and (D). Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 6 Angiograms of the porcine femoral pseudoaneurysm (PSA) (animal no. 6) at day 4 before collagen injection (A) and at day 10 (6 days after collagen injection) (B). Tweezers were positioned on the PSA segment (white arrowheads). In (A), the PSA was open. In (B), it was closed after collagen injection. White arrows indicate the direction of arterial blood flow in the femoral artery (FA). Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 7 Macroscopic image of cross-sectioned pseudoaneurysm (PSA) model, 6 days after collagen injection (animal no. 1, day 10). FA, Femoral artery; PSA, pseudoaneurysm model with thrombus; c, injected type I collagen suspension (4% [w/v] in MilliQ water). The white dashed lines indicate the boundary of the PSA wall. Bar indicates 5 mm. Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 8 Microscopic images of coagulated pseudoaneurysm (PSA) model (luminal site) 6 days after type I collagen injection. Hematoxylin-eosin stained sections showed a clotted PSA filled with type I collagen (c) and coagulated blood cells (black arrows) (A) and (B). The wall of the PSA is indicated by dashed line (A). Leukocytes were found within the collagen network (white arrows) (B). c, Collagen network. Bar indicates 500 μm in (A) and 50 μm in (B). Journal of Vascular Surgery 2010 52, 1330-1338DOI: (10.1016/j.jvs.2010.05.118) Copyright © 2010 Society for Vascular Surgery Terms and Conditions