Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,

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Presentation transcript:

Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD, Ashok Purohit, MD, Frédéric de Blay, MD, Rudolf Valenta, MD, Gabrielle Pauli, MD Journal of Allergy and Clinical Immunology Volume 120, Issue 2, Pages 315-321 (August 2007) DOI: 10.1016/j.jaci.2007.03.046 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Dot-plot representation of the skin reactivities. Mean wheal areas (in square millimeters; y-axis) are displayed for the different concentrations (4, 12, 36, and 108 μg/mL) of the HM, histamine, and the natural grass pollen extract (GP; x-axis). Horizontal lines indicate median values. The natural grass pollen extract induced a significantly larger skin reaction than the HM for the 3 first concentrations (P < .001) and for the highest concentration (P < .004). Journal of Allergy and Clinical Immunology 2007 120, 315-321DOI: (10.1016/j.jaci.2007.03.046) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Dose-response curves of the log of the mean wheal areas (y-axis) obtained with the log of the 4 different concentrations of the HM (x-axis; n = 32). Three of the 32 patients were not tested at 108 μg/mL because they had clearly positive test responses at 36 μg/mL. The calculation of the slope does not differ significantly with or without their results. Journal of Allergy and Clinical Immunology 2007 120, 315-321DOI: (10.1016/j.jaci.2007.03.046) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Dose-response curves of the log of the geometric mean wheal areas of the 32 patients and the 95% confidence limits (y-axis) obtained with the log of the 4 different concentrations of the HM (x-axis). The mean slope was 0.630 (0.370-1.207). Journal of Allergy and Clinical Immunology 2007 120, 315-321DOI: (10.1016/j.jaci.2007.03.046) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Correlation between HM-specific IgE levels (x-axis) and grass pollen extract–specific IgE levels (y-axis). Right, Grass pollen extract–specific IgE levels measured by means of ELISA. Left, Timothy grass pollen–specific IgE levels measured by means of ImmunoCAP (in kilounits per liter). A significant correlation (r = 0.98, P < .0001) was observed with both methods. Journal of Allergy and Clinical Immunology 2007 120, 315-321DOI: (10.1016/j.jaci.2007.03.046) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Box-plot representation of IgE levels. IgE levels (OD values) specific for individual grass pollen allergens, HM, and grass pollen extract (GP) are displayed for the 32 patients on the y-axis. Means and outliers (+), as well as the percentage of positive sera, are shown. Journal of Allergy and Clinical Immunology 2007 120, 315-321DOI: (10.1016/j.jaci.2007.03.046) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Induction of basophil histamine release with the HM. Basophils from 6 representative patients were exposed to increasing concentrations of the HM or anti-IgE (x-axis). The percentages of total histamine release are shown on the y-axis for each of the patients. Journal of Allergy and Clinical Immunology 2007 120, 315-321DOI: (10.1016/j.jaci.2007.03.046) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions