iGEM Advisors Meeting 4/23/2010

First week of summer Project Proposal Where to start research on our project? Biosafety! Regional Conference Update Who is interested in going to the Competition November 5th-7th? Access to IGB

Summer Meetings Tools + Wetlab general stuff– 1/wk Tools + Wetlab Administrative stuff – 1/wk Wetlab + Professors – 1/wk Wetlab – 1/day 10am (except May 23-27th)

May 23rd – 27th Mornings Afternoon – Lab Work! Day 1 Procedure for Intro Project Day 2 How to research Parts Registry Primer Design Day 3 OpenWetWare/Wiki Modeling Basics Day 4 Data Collection Bioethics Day 5 Meetings, etc. Pull Reporters/Promoters/etc. from Parts Registry Standard Assembly Basic modeling Testing Work on Project Procedure

Project Proposal Use pre-existing pathways to make e. coli. Take in metal from the surroundings and reduce it to precipitate metal ores. Focus on two different metals and an alloy of the two Create and a decoder function Using tf, RNA regulation, or both? Combine the decoder and metal reducing pathways to make a cell that will produce either one or the other type of metal, or an alloy when the two are both present. Degredation and Biodegredation; Olefins and Parafins, Aromatic Compounds, Alkanes, Alkenes, Hydrocarbon into Google Scholar.

Project Proposal The 2010 Illinois iGEM Bioware team project involves the construction of a biological system that functions as a decoding nanofabricator. As a continuation of the previous Illinois iGEM Bioware project: The Bacterial Decoder, the decoding bionanofabricator will produce specifically structured, purified, metallic ores depending on the presence of metals in its environment. This system will also possess the capacity to produce metal alloys by analyzing the presence and combination of specific metals in the environment. This system will take advantage of metal-respiring and metal detoxifying microbes that possess the ability to reduce metals as terminal electron acceptors and/or reduce toxic forms of metals into insoluble, harmless forms. The decoding nanofabricator will emphasize the capacity of information processing in biological systems to perform human-define tasks in industrial, manufacturing, computing, and environmental applications.