p110-free p85 is present in MEFs and certain murine tissues.

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Photograph of a Zmpste24−/− mouse, illustrating a characteristic dragging of the hind limb. Bergo M O et al. PNAS 2002;99: ©2002 by National.

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Presentation transcript:

p110-free p85 is present in MEFs and certain murine tissues. p110-free p85 is present in MEFs and certain murine tissues. (A) The absolute amounts of avi-tagged p85s compared with the amount of p110s recovered with them in streptavidin pull-downs from p85αavi/avi or p85β avi/avi MEFs, corrected for input protein (means, ±SD, from two clones/genotype, each clone was measured three times). (B and C) Experiments like those in A except using tissues from mice expressing avi-tagged p85s and mBirA. Data are corrected for input protein (means ± SD, from three independent mice). (D) The percentage of total p110-free p85 in different mouse tissues was calculated from results shown in A–C. Statistically significant at *P < 0.05, **P < 0.01, and ***P < 0.001. NS, not significant. The underlying data are in SI Appendix, Fig. S11. N. Tsolakos et al. PNAS 2018;115:48:12176-12181 ©2018 by National Academy of Sciences