Identification of SERCA2 as a binding protein of toll‐like receptor 9 (TLR9) Representative image of tandem affinity purification visualized by silver.

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Identification of SERCA2 as a binding protein of toll‐like receptor 9 (TLR9) Representative image of tandem affinity purification visualized by silver stain is presented. Identification of SERCA2 as a binding protein of toll‐like receptor 9 (TLR9) Representative image of tandem affinity purification visualized by silver stain is presented. Tandem affinity purification was performed using cardiomyocytes that were adenovirally transfected with TLR9‐HA‐FLAG or YFP‐HA‐FLAG. Cardiac fibroblasts transfected with TLR9‐HA‐FLAG were used as a control. MW: molecular weight markers. Note that the 95‐kDa band marked by an asterisk was observed in TLR9 immunoprecipitates from cardiomyocytes, but not from cardiac fibroblasts. CpG‐ODN increased the target protein binding to TLR9 (asterisk) in cardiomyocytes. Source data are available online for this figure. Yasunori Shintani et al. EMBO Rep. 2014;embr.201337945 © as stated in the article, figure or figure legend