P110-free p85 is recruited to PDGFRs with higher affinity than the p85-p110 dimers at low levels of receptor activation. p110-free p85 is recruited to.

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p110-free p85 is recruited to PDGFRs with higher affinity than the p85-p110 dimers at low levels of receptor activation. p110-free p85 is recruited to PDGFRs with higher affinity than the p85-p110 dimers at low levels of receptor activation. (A) The amounts of p110-free p85 associated with PDGFRs following stimulation with PDGF-BB (1 min). Levels are expressed as a percentage of the total p85 associated with PDGFRs (as presented in Fig. 5). (B) Absolute amounts of p110-free p85 recovered with PDGFRs, quantified by mass spec (“measured”) compared with the amount predicted if no difference in affinity, between p110-free and heterodimeric-p85, existed (“expected”). Expected values were calculated by multiplying the amount of total p85 recruited to PDGFR (Fig. 5C) with the percentage p110-unbound p85 in that clone (Fig. 3D). (C) Amounts of p110-free p85β in streptavidin pull-downs from MEFs expressing p85βavi/avi, with (+) or without (−) prior pull-down of PDGFRs. The amount is expressed as a percentage of the total amount of p85β pulled down or as absolute levels. (D) Estimating the apparent affinity of interaction between recombinant, purified, monomeric p85α or p85α-p110α dimers and PYPs using fluorescence polarization. A given protein preparation was equilibrated with a fluorescein-labeled PYP (Fluor-GpYMDMS, 2 nM) and a doubly phosphorylated, nonfluorescent PYP was titrated into the mixture to the indicated concentrations. The data are all means, ±SD, from three independent experiments. Statistically significant at *P < 0.05 and **P < 0.01. N. Tsolakos et al. PNAS 2018;115:48:12176-12181 ©2018 by National Academy of Sciences