HLA-B∗1502–bound peptides: Implications for the pathogenesis of carbamazepine- induced Stevens-Johnson syndrome Chih-Wen Ou Yang, MS, Shuen-Iu Hung, PhD,

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HLA-B∗1502–bound peptides: Implications for the pathogenesis of carbamazepine- induced Stevens-Johnson syndrome Chih-Wen Ou Yang, MS, Shuen-Iu Hung, PhD, Chiun-Gung Juo, PhD, Ya-Ping Lin, MS, Wu-Hsiang Fang, MS, I.-Hsuan Lu, MS, Shui- Tein Chen, PhD, Yuan-Tsong Chen, MD, PhD Journal of Allergy and Clinical Immunology Volume 120, Issue 4, Pages 870-877 (October 2007) DOI: 10.1016/j.jaci.2007.06.017 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 HLA-B∗1502 peptides identified with liquid chromatography–tandem mass spectrometry. A, Chromatogram illustrates change in the total ion current. B, Ions at mass-to-charge ratio(m/z) 516.1 and 1030.4 were predicted to have the same sequence with different charges. C, Product-ion spectrum of the ion at m/z 516.1. The ion was identified as the peptide labeled with formaldehyde, f-DLAVPHTSY. D, Product-ion spectrum of the same peptide without labeling, DLAVPHTSY. Journal of Allergy and Clinical Immunology 2007 120, 870-877DOI: (10.1016/j.jaci.2007.06.017) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 HLA-B∗1502 peptide-binding assay. A, ILGPPGSVY was the positive control. B, Assays for 5 peptides we and others28 identified (a-e) and 2 serine-rich peptides (f and g). C, Assays for 6 peptides (i-n) and 2 proline-rich peptides (o and p) we identified. In Fig 2, B and C, a peptide derived from the HLA-A2–restricted HBV epitope was the negative control (h). Journal of Allergy and Clinical Immunology 2007 120, 870-877DOI: (10.1016/j.jaci.2007.06.017) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Mass spectra of soluble HLA-B∗1502–bound peptides with (A) and without (B) CBZ. Peptide compositions were compared at 1-minute intervals. Signals between 52 and 53 minutes were compared. To compare the graphs in detail, each graph was divided into 3 or 4 parts, and each part was zoomed out to align the signals (C). Journal of Allergy and Clinical Immunology 2007 120, 870-877DOI: (10.1016/j.jaci.2007.06.017) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions