Proteomics, oxidative stress and male infertility

Slides:

Advertisements

Similar presentations

Advertisements

Chromosomal polymorphisms are associated with female infertility and adverse reproductive outcomes after infertility treatment: a 7-year retrospective.

Sezgin Gunes, Maha Al-Sadaan, Ashok Agarwal

Mostafa Metwally, Cynthia M. Farquhar, Tin Chiu Li

Adverse pregnancy and neo-natal outcomes after assisted reproductive treatment in patients with pelvic endometriosis: a case–control study Marianne Jacques,

Volume 85, Issue 4, Pages (April 2014)

Causes, effects and molecular mechanisms of testicular heat stress

Chromosomal polymorphisms are associated with female infertility and adverse reproductive outcomes after infertility treatment: a 7-year retrospective.

Stem cell therapeutic possibilities: future therapeutic options for male-factor and female- factor infertility? Charles A. Easley, Calvin R. Simerly,

A systematic review of the effect of oral antioxidants on male infertility C. Ross, A. Morriss, M. Khairy, Y. Khalaf, P. Braude, A. Coomarasamy, T. El-Toukhy

Proteomic Serum Profile of Fatigued Men Receiving Localized External Beam Radiation Therapy for Non-Metastatic Prostate Cancer Nada Lukkahatai, PhD,

Attitudes and policies regarding access to fertility care and assisted reproductive technologies in Israel Daniel Sperling, Yael Simon Reproductive.

Oxidative stress and medical antioxidant treatment in male infertility

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification A. Bizzini, G. Greub

Shubhadeep Roychoudhury, Ashok Agarwal, Gurpriya Virk, Chak-Lam Cho

Delphine Haouzi, Hervé Dechaud, Said Assou, John De Vos, Samir Hamamah

Omics as a window to view embryo viability

Role of Withania somnifera (Ashwagandha) in the management of male infertility Pallav Sengupta, Ashok Agarwal, Maria Pogrebetskaya, Shubhadeep Roychoudhury,

E. Jauniaux, I. Ben-Ami, R. Maymon Reproductive BioMedicine Online

E. Jauniaux, I. Ben-Ami, R. Maymon Reproductive BioMedicine Online

Willem Ombelet Reproductive BioMedicine Online

Analytical Characteristics of Cleavable Isotope-Coded Affinity Tag-LC-Tandem Mass Spectrometry for Quantitative Proteomic Studies Cecily P. Vaughn, David.

Tracey A. Edgell, Luk J.F. Rombauts, Lois A. Salamonsen

Single layer centrifugation of stallion spermatozoa improves sperm quality compared with sperm washing J.M. Morrell, H. Rodriguez-Martinez, A. Johannisson

Detection of microorganisms in blood specimens using matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a review M. Drancourt

Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass.

Proteomic analysis of seminal plasma from infertile patients with oligoasthenoteratozoospermia due to oxidative stress and comparison with fertile volunteers

Characterization of lactoferrin receptor on human spermatozoa

Non-invasive prenatal diagnosis using cell-free fetal DNA in maternal plasma from PGD pregnancies Ying Li, Gheona Altarescu, Paul Renbaum, Talia Eldar-Geva,

Comparative analysis of the seminal plasma proteomes of oligoasthenozoospermic and normozoospermic men Elisa Giacomini, Blendi Ura, Elena Giolo, Stefania.

Tanja Burnik Papler, Eda Vrtačnik Bokal, Aleš Maver, Luca Lovrečić

Diagnosis of Aerococcus urinae infections: Importance of matrix-assisted laser desorption ionization time-of-flight mass spectrometry and broad-range.

Why are reproductive cancers more common in nulliparous women?

Proteomics Moves into the Fast Lane

Diagnostic application of oxidation-reduction potential assay for measurement of oxidative stress: clinical utility in male factor infertility Ashok.

Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches Maryam Hezavehei, Mohsen Sharafi, Homa Mohseni Kouchesfahani,

Embryology in the era of proteomics

Barbara Ferreira da Silva, M. Sc

Moniek van der Zanden, Annemiek W. Nap Reproductive BioMedicine Online

A systematic review of the effect of oral antioxidants on male infertility C. Ross, A. Morriss, M. Khairy, Y. Khalaf, P. Braude, A. Coomarasamy, T. El-Toukhy

Hillary B. Alberta, Roberta M. Berry, Aaron D. Levine

What’s on the mind of IVF consumers?

Sezgin Gunes, Maha Al-Sadaan, Ashok Agarwal

F. Bittar, J.-M. Rolain Clinical Microbiology and Infection

The occurrence of Legionella species other than Legionella pneumophila in clinical and environmental samples in Denmark identified by mip gene sequencing.

Effect of energy expenditure and physical activity on the outcomes of assisted reproduction treatment Mert Kucuk, Fadime Doymaz, Bulent Urman Reproductive.

Yaniv Zipori, Jigal Haas, Howard Berger, Eran Barzilay

Mostafa Metwally, Cynthia M. Farquhar, Tin Chiu Li

Identification of diabetes- and obesity-associated proteomic changes in human spermatozoa by difference gel electrophoresis Thomas M Kriegel, Falk Heidenreich,

Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for species identification of Acinetobacter strains isolated.

Pierre P. Massion, MD, Richard M. Caprioli, PhD

Advantages and limitations of genomics in prokaryotic taxonomy

Cumulative delivery rates after ICSI with donor spermatozoa in different age groups Michaël De Brucker, Michel Camus, Patrick Haentjens, Jacques Francotte,

Maternal psychosocial consequences of twins and multiple births following assisted and natural conception: a meta-analysis Olga van den Akker, Gianina-Ioana.

Adverse pregnancy and neo-natal outcomes after assisted reproductive treatment in patients with pelvic endometriosis: a case–control study Marianne Jacques,

Sperm DNA fragmentation index as a promising predictive tool for male infertility diagnosis and treatment management – meta-analyses Daniele Santi, Giorgia.

Should we be promoting embryo transfer at blastocyst stage?

Stephen C. Collins, Esther Chan Reproductive BioMedicine Online

Pietro Gambadauro, Ramesan Navaratnarajah

Implication of apoptosis in sperm cryoinjury

The Alpha Consensus Meeting on the professional status of the clinical embryologist: proceedings of an expert meeting Reproductive BioMedicine Online

C Almeida, M Sousa, A Barros Reproductive BioMedicine Online

Ovarian reserve screening before contraception?

Volume 85, Issue 4, Pages (April 2014)

Association between single-nucleotide polymorphisms of DNMT3L and infertility with azoospermia in Chinese men Jian-Xi Huang, Matthew B. Scott, Xiao-Ying.

Mahesh Mysore Shivananjappa, Muralidhara

Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility Xia Wang, M.D., Ph.D.,

Yaniv Zipori, Jigal Haas, Howard Berger, Eran Barzilay

Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species Reda Mahfouz, M.D., Rakesh.

Western blot analysis of plasma proteins from two-dimensional gel electrophoresis. Western blot analysis of plasma proteins from two-dimensional gel electrophoresis.

Presentation transcript:

Proteomics, oxidative stress and male infertility Ashok Agarwal, Damayanthi Durairajanayagam, Jacques Halabi, Jason Peng, Monica Vazquez-Levin Reproductive BioMedicine Online Volume 29, Issue 1, Pages 32-58 (July 2014) DOI: 10.1016/j.rbmo.2014.02.013 Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Primary, secondary and tertiary forms of ROS and different types of free radicals. Reproductive BioMedicine Online 2014 29, 32-58DOI: (10.1016/j.rbmo.2014.02.013) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Model of the build up of oxidative stress in the semen. The model highlights the imbalance caused by accumulating ROS and depleting antioxidant, which brings about a state of oxidative stress. Various lifestyle and environmental factors along with testicular and seminal sources cause the generation of ROS. Antioxidants comprise both enzymatic and non-enzymatic types. Reproductive BioMedicine Online 2014 29, 32-58DOI: (10.1016/j.rbmo.2014.02.013) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Overview of the general methods used for protein isolation and identification in seminal plasma and spermatozoa. Levels of oxidative stress (OS) in the semen sample are determined by measuring the reactive oxygen species (ROS) and/or the total antioxidant capacity (TAC). Protein separation can be performed using either 2D gel electrophoresis (2D-GE) or 2D differential in-gel electrophoresis (2D-DIGE). Next, protein identification can be done using liquid chromatography-tandem mass spectrometry (LC-MS/MS) or matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) method. Isolated proteins are identified using tandem MS data analysis programs such as MASCOT or SEQUEST. Proteins of interest can be validated using Western blotting. Based on spectral counts, protein expression is quantified. Protein lists are generated and analysed using bioinformatics. Gene ontology (GO) is then used to determine the specific gene function in cellular pathways. Reproductive BioMedicine Online 2014 29, 32-58DOI: (10.1016/j.rbmo.2014.02.013) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions