Role of proton gradients and vacuolar H+-ATPases in the refilling of intracellular calcium stores in exocrine cells  C. Camello, J.A. Pariente, G.M. Salido,

Slides:



Advertisements
Similar presentations
Human Keratinocytes Express Multiple P2Y-Receptors: Evidence for Functional P2Y1, P2Y2, and P2Y4 Receptors  Helen E. Burrell, Wayne B. Bowler, James A.
Advertisements

Volume 137, Issue 4, Pages (October 2009)
Volume 38, Issue 1, Pages (January 2006)
Altered Calcium-Mediated Cell Signaling in Keratinocytes Cultured from Patients with Neurofibromatosis Type 1  Timo Korkiamäki, Heli Ylä-Outinen, Jussi.
Volume 139, Issue 5, Pages e5 (November 2010)
Volume 135, Issue 2, Pages (August 2008)
Negative Feedback Synchronizes Islets of Langerhans
Switching Heterotrimeric G Protein Subunits with a Chemical Dimerizer
Michiko Tashiro, Hana Inoue, Masato Konishi  Biophysical Journal 
Calcium Stores in Hippocampal Synaptic Boutons Mediate Short-Term Plasticity, Store- Operated Ca2+ Entry, and Spontaneous Transmitter Release  Nigel J.
Jean-Yves Chatton, Yumei Cao, Jörg W. Stucki  Biophysical Journal 
Sodium Entry during Action Potentials of Mammalian Neurons: Incomplete Inactivation and Reduced Metabolic Efficiency in Fast-Spiking Neurons  Brett C.
Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft
Martin D Bootman, Michael J Berridge  Current Biology 
Volume 57, Issue 1, Pages (January 2000)
Volume 135, Issue 2, Pages (August 2008)
Volume 38, Issue 2, Pages (April 2010)
Volume 130, Issue 3, Pages (March 2006)
Different Metabolic Responses in α-, β-, and δ-Cells of the Islet of Langerhans Monitored by Redox Confocal Microscopy  Ivan Quesada, Mariana G. Todorova,
Volume 126, Issue 1, Pages (January 2004)
Calcium-Dependent Facilitation and Graded Deactivation of Store-Operated Calcium Entry in Fetal Skeletal Muscle  Claude Collet, Jianjie Ma  Biophysical.
Volume 58, Issue 5, Pages (November 2000)
Lori Redmond, Amir H. Kashani, Anirvan Ghosh  Neuron 
Psoriasis and Altered Calcium Metabolism: Downregulated Capacitative Calcium Influx and Defective Calcium-Mediated Cell Signaling in Cultured Psoriatic.
Volume 122, Issue 7, Pages (June 2002)
Presence of store-operated Ca2+ entry in C57BL/6J mouse ventricular myocytes and its suppression by sevoflurane  A. Kojima, H. Kitagawa, M. Omatsu-Kanbe,
Volume 139, Issue 5, Pages e5 (November 2010)
Volume 81, Issue 1, Pages (January 2014)
Volume 38, Issue 2, Pages (April 2010)
Arterial Myogenic Activation through Smooth Muscle Filamin A
Volume 8, Issue 11, Pages (November 2015)
Lipid Rafts Establish Calcium Waves in Hepatocytes
Regulation of Airway Ciliary Activity by Ca2+: Simultaneous Measurement of Beat Frequency and Intracellular Ca2+  Alison B. Lansley, Michael J. Sanderson 
TRPV3 Channels Mediate Strontium-Induced Mouse-Egg Activation
David Zenisek, Gary Matthews  Neuron 
Volume 9, Issue 16, Pages S1-918 (August 1999)
The Reduced Release Probability of Releasable Vesicles during Recovery from Short- Term Synaptic Depression  Ling-Gang Wu, J.Gerard G Borst  Neuron  Volume.
Long-Range Ca2+ Signaling from Growth Cone to Soma Mediates Reversal of Neuronal Migration Induced by Slit-2  Chen-bing Guan, Hua-tai Xu, Ming Jin, Xiao-bing.
Lysophospholipid Receptor-Mediated Calcium Signaling in Human Keratinocytes  Karin Lichte, Roberto Rossi, Kerstin Danneberg, Michael ter Braak, Ulrich.
Volume 6, Issue 8, Pages (August 1996)
Polycations induce calcium signaling in glomerular podocytes
Volume 58, Issue 6, Pages (June 2008)
Volume 111, Issue 5, Pages (November 2002)
Volume 23, Issue 6, Pages (June 2016)
Volume 19, Issue 19, Pages (October 2009)
Hitoshi Komuro, Pasko Rakic  Neuron 
Chia-Ling Tu, Wenhan Chang, Daniel D. Bikle 
CRAC Channels Drive Digital Activation and Provide Analog Control and Synergy to Ca2+-Dependent Gene Regulation  Pulak Kar, Charmaine Nelson, Anant B.
Stephan D. Brenowitz, Wade G. Regehr  Neuron 
Cell-Specific Manipulation of Second Messengers
Volume 117, Issue 6, Pages (December 1999)
Volume 18, Issue 3, Pages (January 2017)
Volume 79, Issue 1, Pages (July 2000)
Imaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes
Dendritic Integration in Mammalian Neurons, a Century after Cajal
Volume 75, Issue 4, Pages (October 1998)
Ca2+ waves require sequential activation of inositol trisphosphate receptors and ryanodine receptors in pancreatic acini  M.Fatima Leite, Angela D. Burgstahler,
Current Injection Provokes Rapid Expansion of the Guard Cell Cytosolic Volume and Triggers Ca2+ Signals  Lena J. Voss, Rainer Hedrich, M. Rob G. Roelfsema 
Volume 21, Issue 22, Pages (November 2011)
Volume 137, Issue 4, Pages (October 2009)
Susan K. Fellner, William J. Arendshorst  Kidney International 
Ca2+ Flow via Tunnels in Polarized Cells: Recharging of Apical Ca2+ Stores by Focal Ca2+ Entry through Basal Membrane Patch  Hideo Mogami, Kyoko Nakano,
Inositol Trisphosphate and Cyclic ADP-Ribose–Mediated Release of Ca2+ from Single Isolated Pancreatic Zymogen Granules  Oleg V Gerasimenko, Julia V Gerasimenko,
Volume 10, Issue 6, Pages (December 2009)
John R. Henley, Kuo-hua Huang, Dennis Wang, Mu-ming Poo  Neuron 
A Novel Role for Bcl-2 in Regulation of Cellular Calcium Extrusion
Volume 12, Issue 23, Pages (December 2002)
Assessment of Sarcoplasmic Reticulum Ca2+ Depletion During Spontaneous Ca2+ Waves in Isolated Permeabilized Rabbit Ventricular Cardiomyocytes  N. MacQuaide,
ATP-Independent Luminal Oscillations and Release of Ca2+ and H+ from Mast Cell Secretory Granules: Implications for Signal Transduction  Ivan Quesada,
Annexin 5 mediates a peroxide-induced Ca2+ influx in B cells
Presentation transcript:

Role of proton gradients and vacuolar H+-ATPases in the refilling of intracellular calcium stores in exocrine cells  C. Camello, J.A. Pariente, G.M. Salido, P.J. Camello  Current Biology  Volume 10, Issue 3, Pages 161-164 (February 2000) DOI: 10.1016/S0960-9822(00)00313-4

Figure 1 Polarization of Ca2+ signals during CCE. (a) The protocol followed to study the polarization of Ca2+ signals during CCE in the presence of ACh in fura-2-loaded pancreatic acinar cells. After depletion of Ca2+ stores with 10 μM ACh in Ca2+-free medium (1 mM EGTA; left segment of the trace), CCE was initiated by applying 10 mM Ca2+ (right segment of the trace). The gap corresponds to the decay phase of the Ca2+ response, when the record was usually paused. (b) Pseudocolor images showing the high-speed ratio frames obtained during the response to ACh (upper panel) and during CCE (lower panel) at the times indicated in the graphs to the right. Measurements are those obtained from the experiment shown in (a). The [Ca2+]i traces (fura-2 ratio F340/F380) of luminal (L) and basolateral (B) areas of the cells are displayed in blue (luminal) and red or black (basal). The bright-field image shows the luminal pole of the cells as a granular central area. During activation of influx (lower panel), there is a luminal Ca2+ gradient located at the site of origin of the initial response to ACh. (c) Effects of application of 1 μM thapsigargin (TPS) on the luminal Ca2+ gradient occuring during CCE. The top panel shows the initiation of a Ca2+ wave in the luminal pole (L; blue trace on graph) in response to ACh. The lower panel and the accompanying graph demonstrate the lack of a Ca2+ gradient during initiation of CCE. A pseudocolor calibration bar is provided for each group of images. Isolated pancreatic acinar cells loaded with the calcium dye Fura-2 AM (1 μM, 30 min), placed on the stage of a Nikon microscope, were excited at 340 and 380 nm, and the fluorescence emission at 515 nm was recorded with a high-speed digital CCD camera (Hamamatsu, HisCa C-6790), rendering 4–5 ratio images/sec (F340/F380 values, pixel-by-pixel). Current Biology 2000 10, 161-164DOI: (10.1016/S0960-9822(00)00313-4)

Figure 2 Nigericin and folimycin disrupt Ca2+ gradients during influx in cells continuously stimulated with 10 μM ACh. The protocol used was similar to that shown in Figure 1, except that nigericin (10 μM) or folimycin (100 nM) were added before readmission of extracellular calcium. (a) Pseudocolor ratio images obtained during the initial response to ACh (upper panel) and during the subsequent CCE (lower panel) in nigericin-treated cells. The associated graphs show the ratio values for luminal (L; blue trace) and basolateral (B; red trace) areas, displayed in the bright-field image. (b) A similar analysis of the calcium entry phase in folimycin-treated cells. As can be seen, both nigericin and folimycin abolished the Ca2+ gradient that normally occurs during CCE. (c) For comparison, a control experiment, similar to that shown in Figure 1b, was performed. The images correspond to the initial response to ACh (upper panel) and the onset of CCE (lower panel), and the graphs contain the traces for luminal (L; blue) and basolateral (B; red) areas. Current Biology 2000 10, 161-164DOI: (10.1016/S0960-9822(00)00313-4)

Figure 3 A putative model for the transfer of Ca2+ between different Ca2+ stores in exocrine acinar cells. Emptying of intracellular Ca2+ stores by InsP3, formed in response to agonist stimulation, activates capacitative calcium entry (CCE). Ca2+ is then transported into thapsigargin-sensitive stores before it is conveyed to apical stores (probably the zymogen granules), where a part can leak into the cytosol provided that cell stimulation is preserved. Vacuolar H+-ATPases (V) create proton gradients which aid in store refilling and transfer of Ca2+ to the agonist-sensitive pools, possibly by promoting GTP-induced communication between compartments. Nigericin and folimycin inhibit the gradient and thereby impair calcium transfer. Current Biology 2000 10, 161-164DOI: (10.1016/S0960-9822(00)00313-4)