Relationship between DNA damage and sperm head birefringence

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Presentation transcript:

Relationship between DNA damage and sperm head birefringence C.G. Petersen, L.D. Vagnini, A.L. Mauri, F.C. Massaro, M. Cavagna, R.L.R. Baruffi, J.B.A. Oliveira, J.G. Franco Reproductive BioMedicine Online Volume 22, Issue 6, Pages 583-589 (June 2011) DOI: 10.1016/j.rbmo.2011.03.017 Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 (A) The polarization inverted microscope 1 (TE 300; Nikon, Japan) equipped with Hoffman contrast and polarizing lens; (B) Hoffman objective of 20×; (C) C-mount; (D) camera; and (E) 21-inch monitor. Reproductive BioMedicine Online 2011 22, 583-589DOI: (10.1016/j.rbmo.2011.03.017) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 (A) Spermatozoon with total sperm head birefringence (SHBF-T) observed at 2500× magnification; (B) spermatozoon with partial sperm head birefringence (SHBF-P) observed at 2500× magnification. Reproductive BioMedicine Online 2011 22, 583-589DOI: (10.1016/j.rbmo.2011.03.017) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Sperm head birefringence selection and processing for DNA evaluation. AO = acridine orange; PVP = polyvinylpyrrolidone; SHBF-P = partial sperm head birefringence; SHBF-T = total sperm head birefringence; and TUNEL = TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling. Reproductive BioMedicine Online 2011 22, 583-589DOI: (10.1016/j.rbmo.2011.03.017) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions