In ovo method for evaluating the effect of nutritional therapies on tumor development, growth and vascularization  Yves M. Dupertuis, Florence Delie, Marie Cohen, Claude Pichard  Clinical Nutrition Experimental  Volume 2, Pages 9-17 (October 2015) DOI: 10.1016/j.yclnex.2015.08.001 Copyright © 2015 European Society for Clinical Nutrition and Metabolism Terms and Conditions

Fig. 1 Description of the different steps to evaluate the impact of nutritional therapies on the growth and vascularization of tumor spheroids xenografted in the CAM, using microPET/CT. 1) Cancer cell culture in 96-well spheroid formation plate with extracellular matrix; 2) Tumor spheroid grafting in the CAM; 3) Chemotherapy using intravenous injections with/without nutritional therapy and/or radiotherapy using SRT100 Topex irradiator; 4) Evaluation of tumor vascularization using fluorescence microscopy; 5) Evaluation of tumor growth ([18F]FDG) and angiogenesis (VEGF, integrins, a2b3 or a5b1) using microPET/CT imaging. Clinical Nutrition Experimental 2015 2, 9-17DOI: (10.1016/j.yclnex.2015.08.001) Copyright © 2015 European Society for Clinical Nutrition and Metabolism Terms and Conditions

Fig. 2 Changes in tumor size in the CAM at 24 h, 48 h and 72 h after a new treatment (red) compared with shame treatment (blue). Fluorescence microscopy after sulforhodamine injection indicates that the tumor surface growth tends to stop and even decrease three days after treatment, compared to control. Clinical Nutrition Experimental 2015 2, 9-17DOI: (10.1016/j.yclnex.2015.08.001) Copyright © 2015 European Society for Clinical Nutrition and Metabolism Terms and Conditions

Fig. 3 MicroPET/CT imaging in the CAM model after an intravenous injection of 3 MBq [18F]FDG and 50 μl of Telebrix-38. After MicroPET/CT acquisition, the tumor volume and uptake were measured as 87 μl and 7781 KBq/ml. Clinical Nutrition Experimental 2015 2, 9-17DOI: (10.1016/j.yclnex.2015.08.001) Copyright © 2015 European Society for Clinical Nutrition and Metabolism Terms and Conditions