An Investigation of Oil on Coral Beaching Nathalie Betty Cristina Saskia Shannon

Group 3 Control Experiments In these experiments results were taken under the control conditions for the tank. These results will be used to compare to the results obtained in the experimental tank. Temperature: 240C Salinity: 34 ppt Experiments performed: zoozanthellae count, protein assay, and chlorophyll extraction.

Zoozanthellae Count avg of 5 counts of .004m^3 =126.2 zoox .004mm^3  x   1ml            = 4 x 10^-6ml                   1000mm^3 126.2 zoox  =3.16 x 10^7 zoox/ml 4 x 10^-6ml 2ml(total homogenate volume) x 3.16 x 10^7 zoox/ml  = 6.31 x 10^7 zoox zoox density:    6.31x 10^7 zoox= 7.01 x 10^8 zoox/g wet wt                      .09 g wet wt

Protein Assay Protein (mg/mL) ABS 0.25 0.443 0.5 0.721 0.75 1.0785 1 1.405 1.25 1.59 Unknown 0.806 y= 1.2712X + 0.1141 y= .806 By substitution x = .544 mg/mL of protein

Chlorophyll Extraction Wavelength Chlorophyll 665 0.022 647 0.011 630 0.021 Ca= (11.85x.022)-(1.54x.011)-(.08x.021) =.24205 Mg Chl a/m3 = .24205 x 9 / 1x1.6 = 1.351

Water Accommodated Fraction (WAF) Production and Analysis In order to know the concentration of oil to add to tank we first need to how much oil dissolves in water (WAF). Prepared 36 g of Na and 900 mL of tap water. The oil (Quaker State) was then added at a ratio of 9:1 (900ml seawater:100ml oil). The seawater and oil solution was mixed using a magnetic stirrer for 20 hours and left to settle for the next week to use in each experiment.

Today Today we will calculate the WAF. We will also try to begin the experiment by adding a estimated amount of oil and seeing what happens. By taking the 3 test counts we can determine if the added amount of oil was too much or too little.