Review of Castleton Microarray Images and QC

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Presentation transcript:

Review of Castleton Microarray Images and QC Why do we look at this image? -Good for seeing visual defects -Examining Borders, Chip ID, Controls Why do we look at the QC report? Check 3’ to 5’ ratios of housekeeping genes -Scaling factor -Spike in control signal So lets look at the images and see what happened… team by team

CSC1-CONTROL A GOOD !!

CSC2-FERM GOOD !!

CSC3-CONTROL B GOOD !!

CSC4-DMSO A GOOD !!

CSC5-CONTROL A GOOD !!

CSC6-DMSO A No Good-Failed Rxn  Just kidding GOOD !! 

QC Report From Genechip Actin Housekeeping Control 3’-5’ Ratio TATA BP