Project Idea: Biofuels

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Presentation transcript:

Project Idea: Biofuels Stanford iGEM 2009 Presentation by: Suzie + Robert 3/4/09

Biofuels Introduction What makes a good biofuel?!?! Environmentally friendly Mass production - CHEAP! Chemically: High energy density Low water solubility

Biofuels Introduction

Biofuels Introduction As for synthetic biology applications… Most use E. coli or S. cerevisiae as a host to: 1. Engineer amino acid biosynthesis 2. Engineer fatty acid pathways 3. Engineer isoprenoid pathways

Dr. James Liao

Dr. James Liao

3-Methyl-1-Butanol

2-Methyl-1-Butanol

Dr. James Liao

Our Project We would need to engineer a separate amino acid pathway to produce a possible biofuel EX: Serine or alanine Need to ID enzymes that can make this possible: For Dr. Liao, it was KIVD (yeast) and ADH6 (lactococcus lactis)

Five Possible Sub-projects 1. Synthesizers (constructing the pathway) 2. Riboregulators (controlling knockouts) 3. Modelers (Modeling for bottlenecks) 4. Bottleneckers (Building expression system) 5. ANY OF THE ABOVE

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