A, reversed phase HPLC separation and profile of products from HUVEC and human PMN coincubations. A, reversed phase HPLC separation and profile of products.

Slides:

Advertisements

Similar presentations

Preserving Anti-Platelet Activity of Aspirin by Preventing Hydrolysis John C. Somberg*, Vasant V. Ranade*, Imran Qadeer*, Jackie Coleman**, Janos Molnar***

Advertisements

Date of download: 9/18/2016 Copyright © The American College of Cardiology. All rights reserved. From: An endogenous inhibitor of nitric oxide synthase.

Fig. 1. Impact of AA on ROS generation by human spermatozoa

Prostanoid mediators derived from arachidonic acid and sites of drug action. ASA, acetylsalicylic acid (aspirin); LT, leukotriene; NSAID, nonsteroidal.

From: Neurotoxicity of Lidocaine Involves Specific Activation of the p38 Mitogen-activated Protein Kinase, but Not Extracellular Signal–regulated or c-jun.

Figure 1. Typical structure of polysorbate 80.

From: 15(S)-HETE Production in Human Retinal Microvascular Endothelial Cells by Hypoxia: Novel Role for MEK1 in 15(S)-HETE–Induced Angiogenesis Invest.

Representative 10-s EEG recordings from GAERS: during baseline (prior to injection of any drug) (i), following the i.c.v. injection of 4 μl of vehicle.

Supplemental Data Figure 1: Reverse phase-HPLC of atrial extract from subject 3. Each fraction was assayed with three separate immunoassays: N-terminal.

Supplemental Data Figure 2: Reverse phase-HPLC of ventricle extract from subject 3. Each fraction was assayed with three separate immunoassays: N-terminal.

Supplemental Data Figure 3: Reverse phase-HPLC of peripheral venous plasma extract from subject 3. Each fraction was assayed with two separate immunoassays:

TNFR1 and TNFR2 are expressed on native human ASM but activation of TNFR1 mediates ICAM-1 expression in human ASM cells. TNFR1 and TNFR2 are expressed.

Cytokine-induced rapid ICAM-1 expression on human ASM promotes adhesion of activated T lymphocytes. Cytokine-induced rapid ICAM-1 expression on human ASM.

Htr-9 stimulates ICAM-1 gene expression in human ASM cells.

HIV Protease Inhibitors Stimulate Hepatic Triglyceride Synthesis

Volume 119, Issue 3, Pages (September 2000)

Parallels in antimicrobial peptide mechanisms of action and resistance

Requirement for a β subunit for the modulation of whole-cell CaV2

Comparison of the model incorporating daily dose to that incorporating Cmax. Comparison of the model incorporating daily dose to that incorporating Cmax.

Evaluation of models incorporating the base covariates.

The context-sensitive half-times for commonly used intravenous anesthetic drugs. [Reproduced from Reves JG, Glass PSA, Lubarsky DA, McEvoy MD, and Martinez-Ruiz.

Figure 2 Circadian and ultradian fluctuations in corticosterone

Reverse-phase HPLC chromatograms obtained from analysis of a 24-h human urine sample after consumption of cooked watercress (a) and raw watercress (b).

HPLC tracings of resveratrol (RV) and its major sulfate conjugates M4/M5 in the plasma from three subjects. HPLC tracings of resveratrol (RV) and its major.

Retroviral vector propagation in helper cells and structure of a prototypical vector. Retroviral vector propagation in helper cells and structure of a.

Mean (± S.E.) change from baseline in the 6-min walk distance for bosentan (n = 21) and placebo (n = 11) groups. Mean (± S.E.) change from baseline in.

Mechanism of stabilization of the closed conformation of the umami receptor VFTM by IMP (green). Mechanism of stabilization of the closed conformation.

by Jesmond Dalli, and Charles N. Serhan

Total ion current-EMS chromatograms of SB939 and its metabolites after incubation in liver microsomal fractions for 60 min at a concentration of 50 μM.

AZD7762 abrogated the G2 checkpoint in a novel hollow fiber assay.

J Bone Miner Res 2015;30:1553 (Fig. 1)

Structure of solid lipid nanoparticles (SLNs).

Phylogenetic tree of the human class Frizzled receptors FZD1–10 and SMO created with the ClustalW2 software (ver ;

Volume 115, Issue 1, Pages (July 1998)

Alterations in PUFA metabolism and flux through the P450/sEH pathway related to hypoxia and activation of the renin-angiotensin system. Alterations in.

The three primary ADC components that determine which cells are targeted (antibody), how the drug is released (linker/trigger), and the mechanism of action.

Itcoinitcoin ASAS. 4 I4 I,

Volume 13, Issue 11, Pages (November 2006)

Volume 19, Issue 11, Pages (June 2009)

Volume 6, Issue 3, Pages (March 1997)

Mechanisms of H2S release from S-aroylthiooximes (SATOs).

Cytotoxic activities of polymer micelles containing CFZ in H460 (A) and select micelle formulations in RPMI-8226 (B) cell lines. Cytotoxic activities of.

Cytotoxic effects of PM1 and PM2 empty particle controls and coincubation of CFZ with empty particles tested in H460 (A) and RPMI-8226 (B) cells. Cytotoxic.

Calcium channel structure and ligand binding sites.

Geometric mean concentration-time profiles of (A) racemic BUP, R-BUP, and S-BUP; (B) racemic OHBUP, RR-OHBUP, and SS-OHBUP; (C) racemic ERY, SR-ERYHBUP,

The additive model assumes unspecific effects of equal size in both the placebo and drug arm or groups of studies or experiments. The additive model assumes.

Retention times for identification of coproporphyrin.

Volume 95, Issue 5, Pages (November 1998)

Ethanol enhances retinoic acid metabolism into polar metabolites in rat liver via induction of cytochrome P4502E1 Chun Liu, Robert M. Russell, Helmut.

Effects of inhibitors of drug efflux pumps on the penetration of antibiotics into the central nervous system. Effects of inhibitors of drug efflux pumps.

Volume 74, Issue 8, Pages (October 2008)

Metabolic labeling of IGFBP-5 by T47D breast carcinoma cells

(A) Illustration depicting two types of pharmacokinetic instability of an ADC. Conjugating an antibody with drug can accelerate its clearance (green versus.

Marta Soler, Mercedes Camacho, Ricard Solá, Luís Vila

Effects of gemfibrozil on the exposure (area under the plasma drug concentration-time curve) to different CYP2C8 substrate drugs. Effects of gemfibrozil.

Results of thermogravimetric measurements of peptide ghrelin antagonist (GhA) and loaded into PSi microparticles. Results of thermogravimetric measurements.

Typical HPLC determination of d-Asp by the o-phthaldialdehyde-N-acetyl-l-cysteine (OPA-NAC) method in the frog brain. Typical HPLC determination of d-Asp.

Stereoimage of three independent docking simulations of the interaction between clopidogrel acyl 1-β-d-glucuronide and the active site of CYP2C8. Stereoimage.

J Bone Miner Res 2014;doi: /jbmr.2325 (Fig. 2)

Damage-associated molecular pattern (DAMP)-induced activation of Toll-like receptors (TLRs). Damage-associated molecular pattern (DAMP)-induced activation.

CBD elevates AEA levels in poly-(I:C)–stimulated HaCaT cells after 6 hours. CBD elevates AEA levels in poly-(I:C)–stimulated HaCaT cells after 6 hours.

CBD and other phytocannabinoids are not cytotoxic in HaCaT cells.

The CYP2C8 gene is located to the CYP2C gene cluster on chromosome 10.

AEA and URB597 reduce MCP-2, IL-6, and IL-8 levels in poly-(I:C)–stimulated HaCaT cells after 6 hours. AEA and URB597 reduce MCP-2, IL-6, and IL-8 levels.

Involvement of cys298 in the interaction of PGA1 with AKR1B1.

Activation and desensitization of the α7 nAChRs.

Role of CETP in plasma cholesterol transport.

Neighbor joining tree showing relationships between the human cannabinoid CB1 and CB2 receptors and other human rhodopsin α-group type G protein-coupled.

AppA Is a Blue Light Photoreceptor that Antirepresses Photosynthesis Gene Expression in Rhodobacter sphaeroides Shinji Masuda, Carl E. Bauer Cell Volume.

Characterization of CWR22 xenografts and XMRV-related sequences.

Presentation transcript:

A, reversed phase HPLC separation and profile of products from HUVEC and human PMN coincubations. A, reversed phase HPLC separation and profile of products from HUVEC and human PMN coincubations. Confluent HUVECs were exposed to interleukin-1β for 24 h and treated with 500 μM aspirin (ASA) or vehicle for 20 min at 37°C and 20 μM arachidonic acid for 60 s. Each was incubated with fresh isolated PMNs (HUVEC/PMN cell ratio of 1:50) followed by costimulation with 5 μM ionophore A23187 for 30 min at 37°C. B, UV spectra recorded during the elution of indicated peaks I to IV. Reproduced with permission from the National Academy of Sciences (Claria and Serhan, 1995). Giancarlo Folco, and Robert C. Murphy Pharmacol Rev 2006;58:375-388 The American Society for Pharmacology and Experimental Therapeutics