Association, recombination and mapping of eukaryotic genes

Slides:

Advertisements

Similar presentations

Identification of markers linked to Selenium tolerance genes

Advertisements

Mapping in Arabidopsis cont…

Polymorphism & Restriction Fragment Length Polymorphism (RFLP)

Gene Linkage and Genetic Mapping

Chromosome Mapping in Eukaryotes

Linkage, Recombination and Eukaryotic Mapping. Outline Introduction Complete Linkage compared to independent assortment Crossing over with linked genes.

Polymorphisms: Clinical Implications By Amr S. Moustafa, M.D.; Ph.D. Assistant Prof. & Consultant, Medical Biochemistry Dept. College of Medicine, KSU.

GENOME MAPPING Ms.ruchi yadav lecturer amity institute of biotechnology amity university lucknow(up)

Review Problems 1905 William Bateson and R.C. Punnett

Bassem M. Shoucri Bio Sci D145 January 8, Why do we want to map genomes? Clinical applications! – Identify genes causing diseases – Treatment? Compare.

Studying genomes.

A+ --> a- mutation (forward mutation) a- --> a+ reverse mutation (reversion)

4 Gene Linkage and Genetic Mapping. Mendel’s Laws: Chromosomes Homologous pairs of chromosomes: contain genes whose information is often non- identical.

Nucleic Acids 101. Last week’s grand challenge: Reading and interpreting genetic information.

תדירות רקומבינציה מהכלאה עצמית שימוש בכרומוזום Y כ-'בוחן' שימוש באללים 'מולקולרים' שיטות מיפוי נוספות.

Procedures in RFLP. RFLP analysis can detect Point mutations Length mutations Inversions.

Restriction Fragment Length Polymorphisms (RFLPs) By Amr S. Moustafa, M.D.; Ph.D. Assistant Prof. & Consultant, Medical Biochemistry Dept. College of.

Reading the Blueprint of Life

POLYMERASE CHAIN REACTION AMPLIFYING DNA What do you need to replicate DNA? umZT5z5R8.

DNA Technology and Genomics Chapter 20 A. P. Biology Mr. Knowles Liberty Senior High School.

Genomics BIT 220 Chapter 21.

Announcements: Proposal resubmission deadline 4/23 (Thursday).

Studijní obor Bioinformatika. LAST LECTURE SUMMARY.

Quantitative Genetics. Continuous phenotypic variation within populations- not discrete characters Phenotypic variation due to both genetic and environmental.

Complex Traits Most neurobehavioral traits are complex Multifactorial

Quantitative Genetics

Linkage and Mapping. Figure 4-8 For linked genes, recombinant frequencies are less than 50 percent.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Chapter 5 The Content of the Genome 5.1 Introduction genome – The complete set of sequences in the genetic material of an organism. –It includes the.

ABC for the AEA Basic biological concepts for genetic epidemiology Martin Kennedy Department of Pathology Christchurch School of Medicine.

1 DNA Polymorphisms: DNA markers a useful tool in biotechnology Any section of DNA that varies among individuals in a population, “many forms”. Examples.

Class 22 DNA Polymorphisms Based on Chapter 10 Recombinant DNA Technology Copyright © 2010 Pearson Education Inc.

Molecular markers Non-PCR based 1courtesy of Carol Ritland.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

In The Name of GOD Genetic Polymorphism M.Dianatpour MLD,PHD.

Simple-Sequence Length Polymorphisms SSLPs Short tandemly repeated DNA sequences that are present in variable copy numbers at a given locus. Scattered.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Types of genome maps Physical – based on bp Genetic/ linkage – based on recombination from Thomas Hunt Morgan's 1916 ''A Critique of the Theory of Evolution'',

DNA Fingerprinting Maryam Ahmed Khan February 14, 2001.

4 Gene Linkage and Genetic Mapping

8 and 11 April, 2005 Chapter 17 Population Genetics Genes in natural populations.

AP EXAM REVIEW SESSION MOLECULAR GENETICS Be prepared, not scared!

Simple-Sequence Length Polymorphisms

Genetic markers and their detection

DNA Forensic Analysis Biotechnology.

DNA Marker Lecture 10 BY Ms. Shumaila Azam

Chapter 4 “DNA Finger Printing”

Restriction Enzymes and Plasmid Mapping

Introduction to bioinformatics lecture 11 SNP by Ms.Shumaila Azam

Relationship between Genotype and Phenotype

Relationship between Genotype and Phenotype

Relationship between Genotype and Phenotype

Genetics and Biometrics

Gene Linkage and Genetic Mapping

Gene Linkage and Genetic Mapping

Gene Linkage and Genetic Mapping

Genetic Mapping November 15, 2017.

With Genetic Analyzers from Applied Biosystems

DNA Polymorphisms: DNA markers a useful tool in biotechnology

تهیه کننده بهارا رستمی نیا بهار 94

Mapping Eukaryote Chromosomes by Recombination

Introduction to Genetic Analysis

Sequential Steps in Genome Mapping

Gene mapping March 3, 2017.

The Content of the Genome

9-3 DNA Typing with Tandem Repeats

Relationship between Genotype and Phenotype

Presentation transcript:

Association, recombination and mapping of eukaryotic genes

First genetic map of Sturtevant

TETRADI ORDINATE

Neri-ali norm grigi-ali vest. P bb/vg+vg+ b+b+/vgvg F1 grigi-ali norm (b+b/vg+vg) Reincrocio b+b/vg+vg X bb/vgvg 283 grigio, ali norm. 1294 grigio, ali vest. 1418 nero, ali norm. 241 nero, ali vest.

X Frequency of recombination= number of recombinants 100 number of the offspring X

1. Verifica concatenzaione attraverso reincrocio 2. Ordine dei geni tramite analisi doppio X-over

Frequency of recombination Distance p-j SRec I + DRec Total offspring X 100% (52+46) +(4+2) 500 X 100% = 20.8%= 20.8 u.m. r + Distance j-r SRec II x + DRec Total offspring X 100% (22+22) +(4+2) 500 X 100% = 10%= 10 u.m. Distance p-r SRec I + SR II + 2x DRec Total offspring X 100% (52+46) +(22+22) +2x(4+2) 500 X 100% = 30.8%

Distance between two genes is a map distance (um) 1 um = interval with 1% of recombination Um = cM centiMorgan

MITOTIC CROSSING-OVER (RARE)

Mapping by deletion

Genetic markers (Molecular genetics) RFLP (restriction fragment length polymorphism) VNTR (variable number of tandem repeat) SNP (single nucleotide polymorphism)