T. Maerz, M. D. Newton, K. Kristof, O. Motovylyak, J. S. Fischgrund, D

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Presentation transcript:

Three-dimensional characterization of in vivo intervertebral disc degeneration using EPIC-μCT T. Maerz, M.D. Newton, K. Kristof, O. Motovylyak, J.S. Fischgrund, D.K. Park, K.C. Baker Osteoarthritis and Cartilage Volume 22, Issue 11, Pages 1918-1925 (November 2014) DOI: 10.1016/j.joca.2014.08.006 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Non-contrast-enhanced IVD (A); Contrast-enhanced IVD (B), with the edges of the IVD shown by a dotted line; Distributions of whole IVD attenuation in contrast-enhanced IVD compared to non-contrast-enhanced IVD demonstrates a pronounced shift in the distribution of attenuation data without overlapping with the attenuation spectrum of bone (C). Osteoarthritis and Cartilage 2014 22, 1918-1925DOI: (10.1016/j.joca.2014.08.006) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Height maps calculated from segmented IVDs. 3D segmentation enabled accurate calculations of height maps to analyze zonal differences in disc height. Both disc height and disc volume calculated from segmented EPIC-μCT data sets correlated significantly with disc height measured on histology. Osteoarthritis and Cartilage 2014 22, 1918-1925DOI: (10.1016/j.joca.2014.08.006) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Rutges histologic score (A) and disc height measured on contrast-enhanced μCT (B) as a function of post-operative time point and treatment group. Nucleotomy exhibited significant increases in histologic score at both 3- and 6-week time points. Puncture exhibited significant increases in histologic score only at 6 weeks. Disc height decreased globally in both Puncture and Nucleotomy at both time points, but the largest decreases were evident in the Nucleotomy group, most notably in the posterior zone. * indicates P < 0.05. n = 6 and n = 4 for 3 week Puncture and Nucleotomy, respectively. n = 6 and n = 5, for 6 week Puncture and Nucleotomy, respectively. Error bars represent 95% confidence interval. Osteoarthritis and Cartilage 2014 22, 1918-1925DOI: (10.1016/j.joca.2014.08.006) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Contrast-enhanced attenuation changes due to in vivo Nucleotomy and Puncture. There were significant increases attenuation compared to internal controls in the Nucleotomy group at both 3 and 6 weeks (A). Normalized histograms indicate a shift in attenuation data due to in vivo degeneration, most notably in the Nucleotomy group (B). * indicates P < 0.05. n = 6 and n = 4 for 3 week Puncture and Nucleotomy, respectively. n = 6 and n = 5, for 6 week Puncture and Nucleotomy, respectively. Error bars represent 95% confidence interval. Osteoarthritis and Cartilage 2014 22, 1918-1925DOI: (10.1016/j.joca.2014.08.006) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Scatter plots of histologic disc height vs whole IVD attenuation (A) and μCT disc volume vs whole IVD attenuation (B). IVD attenuation correlated significantly with both disc height and disc volume (n = 48). Osteoarthritis and Cartilage 2014 22, 1918-1925DOI: (10.1016/j.joca.2014.08.006) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Contrast-enhanced μCT colormaps (A–C), Saf-O-stained histologic sections (D–F) and H&E-stained histology sections (G–I) of representative control, Puncture, and Nucleotomy IVDs. Decreased disc height and regions of increased attenuation can be observed in μCT colormaps of both the Puncture and Nucleotomy discs. These regions correspond to the loss of disc height, loss of a healthy NP morphology, and changes in staining intensity indicative of degeneration observed in histologic sections. Osteoarthritis and Cartilage 2014 22, 1918-1925DOI: (10.1016/j.joca.2014.08.006) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions