Despite a relatively small amount of PI3Kβ being associated with activated PDGFRs, it generates a more substantial proportion of the PIP3 that accumulates.

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Despite a relatively small amount of PI3Kβ being associated with activated PDGFRs, it generates a more substantial proportion of the PIP3 that accumulates. Despite a relatively small amount of PI3Kβ being associated with activated PDGFRs, it generates a more substantial proportion of the PIP3 that accumulates. (A) Inhibition of PDGF-stimulated (PDGF-BB, 10 ng/mL, 1 min) PIP3 accumulation by p110α-selective (BYL-719) and/or p110β-selective (TGX-221) inhibitors in MEFs. The data are means, ±SD, from three experiments. (B) Impact of TGX-221 on PIP3 levels following stimulation with various doses of PDGF-BB for 1 min. The data are means, ±SD, from three experiments. (C) Impact of TGX-221 on phosphorylation of PKB following stimulation with various doses of PDGF-BB for 1 min assessed by immunoblotting with anti-phospho- T308 or S473-antibodies. Results were corrected for cell input using βCOP and expressed as percentages of P-PKB following 10 ng/mL PDGF-BB. The data are means, ±SEM, from two experiments. *P < 0.05, **P < 0.01 (t test, two-sample equal variance, two-sided distribution). N. Tsolakos et al. PNAS 2018;115:48:12176-12181 ©2018 by National Academy of Sciences