by Wen-feng Xu, Zhi-wei Xie, Dominic W. Chung, and Earl W. Davie

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by Wen-feng Xu, Zhi-wei Xie, Dominic W. Chung, and Earl W. Davie A Novel Human Actin-Binding Protein Homologue That Binds to Platelet Glycoprotein Ib by Wen-feng Xu, Zhi-wei Xie, Dominic W. Chung, and Earl W. Davie Blood Volume 92(4):1268-1276 August 15, 1998 ©1998 by American Society of Hematology

cDNA clones that span the full-length actin-binding protein homologue sequence. cDNA clones that span the full-length actin-binding protein homologue sequence. Clone K1.3 was isolated by the yeast two-hybrid screening, clones 1-4 were obtained by rapid amplification of 5′ cDNA ends (5′ RACE), and clones 5 and 6 were the cDNA clones isolated from a human placenta library. The diagram shows an alignment of the ABP cDNA with characteristic functional domains of the ABP subunit. The asterisk indicates the position of the 24 amino acid Hinge I insertion in ABP-278. Wen-feng Xu et al. Blood 1998;92:1268-1276 ©1998 by American Society of Hematology

Wen-feng Xu et al. Blood 1998;92:1268-1276 ©1998 by American Society of Hematology

Wen-feng Xu et al. Blood 1998;92:1268-1276 ©1998 by American Society of Hematology

Comparison of ABP-278 and ABP-276 amino terminal 248 amino acids with the actin-binding domains of other ABPs. Amino acids conserved in five or more members of the group are highlighted in bold. Comparison of ABP-278 and ABP-276 amino terminal 248 amino acids with the actin-binding domains of other ABPs. Amino acids conserved in five or more members of the group are highlighted in bold. The group of ABPs includes: human β-spectrin (HSpec),34 Drosophilaspectrin (DrSpec),35 human dystrophin (HDys),37 chicken dystrophin (CDys),36 chicken -actinin (CAct),32 Dictyostelium -actinin (DAct),33 Dictyostelium gelation factor (DGF),31 human ABP-280,17chicken filamin protein (MCFil),47 and human ABP homologues ABP-276 and ABP-278. Wen-feng Xu et al. Blood 1998;92:1268-1276 ©1998 by American Society of Hematology

Alignment of the 24 internal repeats in the ABP-276 and ABP-278 backbone. Alignment of the 24 internal repeats in the ABP-276 and ABP-278 backbone. Alignment of amino acid residues 249-2602 of ABP-278 are shown. The 24 amino acid Hinge I sequence of ABP-278 was inserted between repeats 15 and 16; a 34 amino acid Hinge II sequence of both ABP-276 and ABP-278 was inserted between repeats 23 and 24. A consensus sequence for the repeating unit in ABP-278, derived from residues common to at least 10 of the repeats is listed at the bottom, together with a similar consensus sequence from ABP-28017 and chicken filamin (MCFil).47 Wen-feng Xu et al. Blood 1998;92:1268-1276 ©1998 by American Society of Hematology

Alternative splicing of the Hinge I region. Alternative splicing of the Hinge I region. (A) Schematic representation of the genomic structure encoding the Hinge I region and the PCR products amplified with specific primers P3, P4, P5, and P6. The genomic sequences encoding the Hinge I region and partial sequences of adjacent repeats 15 and 16 are boxed. Intron sequences are represented by dashed lines. The PCR primers specific for ABP-278 (P3, P4, P5, and P6) are labeled. The position of exon-intron junctions are indicated by vertical arrows and numbered. (B) Diagram of the formation of ABP-278 and its isoform ABP-276 by alternative RNA splicing. (C) Partial sequences of exon-intron junctions as numbered in (A). Splice juntions are indicated by slash. The consensus donor (gt) and acceptor (ag) dinucleotides are underlined. Wen-feng Xu et al. Blood 1998;92:1268-1276 ©1998 by American Society of Hematology

Tissue distribution of ABP-276 and ABP-278 by RT-PCR. Tissue distribution of ABP-276 and ABP-278 by RT-PCR. PCR products, amplified with primers P3 and P4 using various marathon cDNAs (Clontech) as templates, were fractionated on 1.5% agarose gel. The upper band (349 bp) represents the PCR products of ABP-278, while the lower band (277 bp) represents the PCR products of ABP-276. The difference in size is due to the presence and absence of the 72 bp exon sequence encoding the 24 amino acid Hinge I region. The PCR amplification of ABP-280 (P9 and P10) and G3PDH (P7 and P8) sequences were used as controls. Wen-feng Xu et al. Blood 1998;92:1268-1276 ©1998 by American Society of Hematology