SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI 603408 cross. SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI 603408.

Slides:



Advertisements
Similar presentations
The roles of IGF-I and IGFBP-3 in the regulation of proximal tubule, and renal cell carcinoma cell proliferation  Catherine W. Cheung, David A. Vesey,
Advertisements

Suppressive Effects of Cyclosporin A and FK-506 on Superoxide Generation in Human Polymorphonuclear Leukocytes Primed by Tumor Necrosis Factor α  Yasushi.
A Naturally Occurring Mutation in FcγRIIA: A Q to K127 Change Confers Unique IgG Binding Properties to the R131 Allelic Form of the Receptor by Cynthia.
by Jun Chung, Xue-Qing Wang, Frederik P. Lindberg, and William A
A Common Genetic Polymorphism (46 C to T Substitution) in the 5′-Untranslated Region of the Coagulation Factor XII Gene Is Associated With Low Translation.
von Willebrand factor is a cofactor in complement regulation
Characterization of assembly of recombinant type IV collagen α3, α4, and α5 chains in transfected cell strains  Takehiro Kobayashi, Makoto Uchiyama  Kidney.
Overlay of two separate 2D gels of soybean seed proteins using Delta2D software. Overlay of two separate 2D gels of soybean seed proteins using Delta2D.
by Yuka Nagata, Eisuke Nishida, and Kazuo Todokoro
Volume 129, Issue 2, Pages (August 2005)
Protease digestion of the purified major allele MBP-CBS fusion protein
nramp2 Mutants Are Hypersensitive to Mn Deficiency.
TEV protease elution from cellulose is specific and provides highly purified target protein. TEV protease elution from cellulose is specific and provides.
The ΔrlmA mutant strain has impaired CWI pathway activation.
Nested Association Mapping (NAM) population design.
Pnut is phosphorylated during early Drosophila embryogenesis.
Mechanism of microthrombosis in HUS
Volume 64, Issue 2, Pages (August 2003)
Juan Codina, Jian Li, Thomas D. Dubose  Kidney International 
Comparative proteomic analysis of human adenomyosis using two-dimensional gel electrophoresis and mass spectrometry  Haiyuan Liu, M.D., Jinghe Lang, M.D.,
Evidence for low-density lipoprotein–induced expression of connective tissue growth factor in mesangial cells  Mimi Sohn, Yan Tan, Richard L. Klein, Ayad.
Basic SNP characteristics using different filter settings for missing data. Basic SNP characteristics using different filter settings for missing data.
Differential conjugation of endogenous SUMO-1 and endogenous SUMO-2/3 to target proteins.A and B, SUMO-1 and SUMO-2 proteins were produced in E coli and.
SDS-PAGE analysis of mature seed from lines Patriot and PI
Renal phenotype is exacerbated in Os and lpr double mutant mice
Figure 4 Glatiramer acetate treatment negatively regulates IFN-β production by targeting components of the IFN-β enhanceosome (A) Wild-type (WT) monocytes.
Seed set of 2xuief, 4xaly, and 4xaly 2xuief mutants in comparison to that of Col-0. Seed set of 2xuief, 4xaly, and 4xaly 2xuief mutants in comparison to.
Multiple distinct molecular mechanisms influence sensitivity and resistance to MDM2 inhibitors in adult acute myelogenous leukemia by Jianting Long, Brian.
The roles of IGF-I and IGFBP-3 in the regulation of proximal tubule, and renal cell carcinoma cell proliferation  Catherine W. Cheung, David A. Vesey,
A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.
Fig. 1. Thorase variants show defects in Thorase oligomer and GluA2-GRIP1 complex disassembly. Thorase variants show defects in Thorase oligomer and GluA2-GRIP1.
Thijn R Brummelkamp, René Bernards, Reuven Agami  Cancer Cell 
2-Methyl-3-Hydroxybutyryl-CoA Dehydrogenase Deficiency Is Caused by Mutations in the HADH2 Gene  Rob Ofman, P.N. Jos Ruiter, Marike Feenstra, Marinus.
Prevalence of Antibodies Against Virus-Like Particles of Epidermodysplasia Verruciformis-Associated HPV8 in Patients at Risk of Skin Cancer  Sabine Stark,
Critical role for Nef in HIV-1–induced podocyte dedifferentiation
Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27  M. Garmyn, A. Pupe  Journal of Investigative Dermatology 
John H. Wiessner, Linda Y. Hung, Neil S. Mandel  Kidney International 
Analysis of GFP expression in gfp loss-of-function mutants.
Md Nasimuzzaman, Danielle Lynn, Johannes CM van der Loo, Punam Malik 
Apolipoprotein E is Present in Primary Localized Cutaneous Amyloidosis
IgG Autoantibodies from Bullous Pemphigoid (BP) Patients Bind Antigenic Sites on Both the Extracellular and the Intracellular Domains of the BP Antigen.
International Society of Nephrology
Immunoblot analysis of GpGCS1 expression in strains K41 (mating-type plus) and K34 (mating-type minus). Immunoblot analysis of GpGCS1 expression in strains.
Purity of Lnp and its derivatives.
Immunoblots of PLCβ isoforms in wild-type and PLCβ2/β3-null platelets.
Fig. 2. Sera IgE reactivity patterns and the frequency of reactivity to raw and cooked shrimp extracts by Western blot. (A, C) All sera were diluted at.
Stomatal aperture. Stomatal aperture. A, Stomata of the different lines with (+) and without (-) ABA treatment as photographed during the experiments.
Figure 2 Glatiramer acetate treatment induced M2 differentiation through a MyD88-independent pathway (A) As described previously,3 M2 monocytes were treated.
Time-dependent phosphorylation profile of p38-MAPK and JNKs in samples from gills of Mytilus galloprovincialis specimens subjected to hyperthermia (30°C).
Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.
Eff localizes in many bands and interbands of wild-type polytene chromosomes. Eff localizes in many bands and interbands of wild-type polytene chromosomes.
Volume 56, Pages S254-S255 (July 1999)
The Asf1–histone H3 interaction is reduced in Asf1-185T and 152T mutants. The Asf1–histone H3 interaction is reduced in Asf1-185T and 152T mutants. The.
Mariangela Marques, Yong Pei, Michael D. Southall, John M
Protease digestion of seven purified MBP-CBS protein alleles.
IRS-1 and -2 expression in four mesangial cell lines from different D-NOD (A and B) and ND-NOD (C and D) mice. IRS-1 and -2 expression in four mesangial.
Urinary cell casts, lymph node weights and proteinuria in the lpr-water, lpr-CU and lpr-TU groups of mice. Urinary cell casts, lymph node weights and proteinuria.
Volume 71, Issue 11, Pages (June 2007)
Mutant MFN2 proteins have normal half-lives and are properly localized to mitochondria. Mutant MFN2 proteins have normal half-lives and are properly localized.
Purification scheme. Purification scheme. (A) Line drawing of CelTag fragment from CelTag plasmid. PCR (polymerase chain reaction) of fragment was performed.
Suppressive Effects of Cyclosporin A and FK-506 on Superoxide Generation in Human Polymorphonuclear Leukocytes Primed by Tumor Necrosis Factor α  Yasushi.
Western immunoblot of Acanthamoeba whole-cell lysates reacted with normal human serum, demonstrating immunoreactivity against Acanthamoeba antigens. Western.
GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila. GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila.
Figure 2. Histoimmunoprecipitation and antigen identification
Epitope-tagged rice RPL18 assembles into functional ribosomes that can be purified by TRAP. (A) Confirmation of RPL18 assembly into ribosomes. Epitope-tagged.
SDS-PAGE and Immunoblot Analyses of Cucurbita PP1 and PP2 in Phloem Exudates of 13-Day-Old Intergeneric Grafts of Cucumiss ativus on Cucurbita maxima.(A)
Effect of LPS on ERK1 and ERK2 activation in human monocytes.
MED25 and JAZ7 Compete to Interact with MYC2.
Expression of MtrE by wild-type and mtr120 mutant strains.
MTB1 Interacts with MYC2 and Disrupts MED25-MYC2 Interaction.
Presentation transcript:

SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI 603408 cross. SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI 603408 cross. (A) SDS-PAGE analysis of total seed proteins and (B) immunoblot analysis of total seed proteins with anti-glycinin 5 antibodies. Samples 1, 13, and 14 are from line Patriot and sample 2 is seed from PI 603408 plants. Samples 3–12 are selected progeny from a 10/81b × PI 603408 cross. The genotype of plant that produced seed is indicated below figure: “+” indicates homozygosity for wild-type alleles, “H” indicates heterozygosity, and “−” indicates homozygosity for mutant alleles. SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis. Jason D. Gillman et al. G3 2017;7:2345-2352 ©2017 by Genetics Society of America