Introduction to the Microscope Lab

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Presentation transcript:

Introduction to the Microscope Lab

Common Microscope Types Compound Monocular Dissecting

Microscope Type Uses Compound Light: Dissection: Used to examine materials mounted on microscope slides (usually sectioned & stained) Generally provides total magnification from 40X to 1000X Does not provide space for dissection of materials being examined. Dissection: Used to examine thick materials on microscope slides & whole specimens living or preserved Generally provides total magnification from 10X to 45X Provides space between stage & objectives for dissection.

Compound Light Microscope Parts & Functions Eyepiece: The part of the microscope you look into Magnifies material being viewed by 10X Sometimes contains a pointer that can be seen as you look into the eyepiece. Also be called the ocular.

Microscope Parts & Functions Nose piece: Part of microscope to which the objectives are attached Rotates to allow for the changing of objectives to increase or decrease magnification.

Microscope Parts & Functions Arm: Where the nosepiece is attached Hold the microscope by this when carrying. Always use both hands!

Microscope Parts & Functions Objectives: Magnify material being examined Scanning objective magnifies 4X (40x) Medium Objective. magnifies 10X (100X) High Powered Obj. magnifies 40X (400x) Total magnification = Eyepiece Mag. X Objective Mag.

Microscope Parts & Functions Stage: Platform on which microscope slide rests Stage clips: Secures slide to the mechanical stage Be careful – if allowed to snap down, they break slides!

Microscope Parts & Functions Coarse adjustment knob: Coarse focusing Used to find specimen, but with less detail. Fine adjustment knob: Precise focusing Small turns make big changes. * Remember – Specimens are 3-Dimensional and focusing adjusts the level you look at.

Microscope Parts & Functions Diaphragm: regulates the amount of light passing through the specimen When viewing translucent specimens, use small opening. When viewing opaque specimens us large opening.

Microscope Parts & Functions Illuminator Light source On/Off switch is on base Base: provides support for microscope

Microscope Storage Always with one hand on the arm & one on the base. Microscope Cabinet Always with one hand on the arm & one on the base.

Preparation for Storage Make sure no slides are on the stage Lower stage for maximum distance between objective & stage Wrap cord around base (clockwise)

Preparation for Use Transport microscope to work station Plug in microscope Inspect and clean microscope

References Robert F. Allen, Professor of Biology Introduction to the Microscope Lab